SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRPL16B
Systematic nameYNL069C
AliasRP23, RPL21B
Description60S ribosomal protein L16-B

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]

Cross References
Swiss-Prot accessionP26785
SGD YNL069C
Gene Ontology (GO)
Cellular componentGO:0005842cytosolic large ribosomal subunit (sensu Eu...TAS:SGD.
Molecular functionGO:0003723RNA bindingIDA:SGD.
Molecular functionGO:0003735structural constituent of ribosomeTAS:SGD.
Biological processGO:0006412translationTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
2N-acetylserine, partial.
43Phosphoserine.
179Phosphoserine.
181Phosphoserine.
185Phosphoserine.
187Phosphoserine.
195Phosphoserine.

Sequence Information
Sequence length198
Molecular weight22249

	---------+----------+----------+----------+----------+
MSQPVVVIDA KDHLLGRLAS TIAKQVLNGQ KIVVVRAEAL NISGEFFRNK   50
LKYHDFLRKA TAFNKTRGPF HFRAPSRILY KAIRGMVSHK TARGKAALER   100
LKIFEGIPPP YDKKKRVVVP QALRVLRLKP GRKYTTLGKL STSVGWKYED   150
VVAKLEDKRK VRSAEYYAKK RAFTKKVSSA SAAASESDVA KQLASFGY     198