SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRPL12B
Systematic nameYDR418W
AliasRPL15A
Description60S ribosomal protein L12

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP17079
SGD YDR418W
Gene Ontology (GO)
Cellular componentGO:0005842cytosolic large ribosomal subunit (sensu Eu...TAS:SGD.
Molecular functionGO:0003735structural constituent of ribosomeTAS:SGD.
Biological processGO:0000027ribosomal large subunit assembly and mainte...IMP:SGD.
Biological processGO:0000723telomere maintenanceIMP:SGD.
Biological processGO:0006412translationTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
4N6,N6-dimethyllysine.
11N6,N6,N6-trimethyllysine, by RKM2.
38Phosphoserine.
67N5-methylarginine, by RMT2.

Sequence Information
Sequence length165
Molecular weight17823

	---------+----------+----------+----------+----------+
MPPKFDPNEV KYLYLRAVGG EVGASAALAP KIGPLGLSPK KVGEDIAKAT   50
KEFKGIKVTV QLKIQNRQAA ASVVPSASSL VITALKEPPR DRKKDKNVKH   100
SGNIQLDEII EIARQMRDKS FGRTLASVTK EILGTAQSVG CRVDFKNPHD   150
IIEGINAGEI EIPEN                                         165