SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRPH1
Systematic nameYER169W
DescriptionDNA damage-responsive transcriptional repressor RPH1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP39956
SGD YER169W
Gene Ontology (GO)
Cellular componentGO:0005634nucleusIC:SGD.
Molecular functionGO:0051864histone demethylase activity (H3-K36 specific)IDA:SGD.
Molecular functionGO:0032453histone demethylase activity (H3-K4 specific)IMP:SGD.
Molecular functionGO:0032454histone demethylase activity (H3-K9 specific)IDA:SGD.
Molecular functionGO:0016566specific transcriptional repressor activityIDA:SGD.
Biological processGO:0006281DNA repairIMP:SGD.
Biological processGO:0016577histone demethylationIDA:SGD.
Biological processGO:0000122negative regulation of transcription from R...IDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
412Phosphoserine.
459Phosphoserine.
557Phosphoserine.
561Phosphoserine.
652Phosphoserine.
689Phosphoserine.

Sequence Information
Sequence length796
Molecular weight90211

	---------+----------+----------+----------+----------+
MTKLIAPSEI VGGVPVFKPT YEQFEDFYAY CKAINKYGMK SGVVKVIPPK   50
EWKDKLDLPY SAETLQKIKI KSPIQQHISG NKGLFMVQNV EKNKTYNIIQ   100
WKDLSKDYVP PEDPKARRNS RKGSVSKSTK LKLKNFESSF NIDDFEQFRT   150
EYTIDLSDFQ NTERLKFLEE YYWKTLNFTT PMYGADTPGS IFPEGLNVWN   200
VAKLPNILDH METKVPGVND SYLYAGLWKA SFSWHLEDQD LYSINYIHFG   250
APKQWYSIPQ EDRFKFYKFM QEQFPEEAKN CPEFLRHKMF LASPKLLQEN   300
GIRCNEIVHH EGEFMITYPY GYHAGFNYGY NLAESVNFAL EEWLPIGKKA   350
GKCHCISDSV EIDVKKLAKS WRDNNKESKG TPPLNQLPNP AMPLLHRPTL   400
KEMESSSLRS TSPDVGHFSN FKSKSSGVSS PLLSRMKDYS NIVEPTLEDP   450
TLKLKRISSF QEQPLNKLLK RETSQTAMLT DHEDNIVAMS LTSMANSAAS   500
SPRLPLSRLN SSNELSNAQP LLDMTNNTLA FPRPNGPSGL NPLLYISNKN   550
ISGISHSAPH SPVNPNISLI KRVKSPNIVT LNISRESSRS PIALNYEARQ   600
QHSQQHSFST PSTVSNLSTS VLGPLSDTND IKTPHPERPN HKTANRILKK   650
ESPVETSKSN LILSKVASTR QEDSFTSRND DLDKEQGSSP LNSKFAPEEI   700
VLSGKNKIYI CKECQRKFSS GHHLTRHKKS VHSGEKPHSC PKCGKRFKRR   750
DHVLQHLNKK IPCISNETTV DAPIMNPTVQ PQDGKAAINQ QSTPLN       796