SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRPF2
Systematic nameYKR081C
DescriptionRibosome biogenesis protein RPF2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]

Cross References
Swiss-Prot accessionP36160
SGD YKR081C
Gene Ontology (GO)
Cellular componentGO:0005730nucleolusIDA:SGD.
Molecular functionGO:00080975S rRNA bindingIPI:SGD.
Molecular functionGO:00083127S RNA bindingIPI:SGD.
Molecular functionGO:0019843rRNA bindingIPI:SGD.
Biological processGO:0030489processing of 27S pre-rRNAIMP:SGD.
Biological processGO:0000027ribosomal large subunit assembly and mainte...IMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
73Phosphoserine.

Sequence Information
Sequence length344
Molecular weight39599

	---------+----------+----------+----------+----------+
MIRTVKPKNA RAKRALVKRE AKLVENVKQA LFIPGQSCNK NLHDIMVDLS   50
ALKKPDMKRF NRKNDIHPFE DMSPLEFFSE KNDCSLMVLM TSSKKRKNNM   100
TFIRTFGYKI YDMIELMVAD NFKLLSDFKK LTFTVGLKPM FTFQGAAFDT   150
HPVYKQIKSL FLDFFRGEST DLQDVAGLQH VISMTIQGDF QDGEPLPNVL   200
FRVYKLKSYK SDQGGKRLPR IELVEIGPRL DFKIGRIHTP SPDMVTEAHK   250
KPKQLEMKTK KNVELDIMGD KLGRIHMGKQ DLGKLQTRKM KGLKSKFDQG   300
TEEGDGEVDE DYEDEASYSD DGQEYEEEFV SATDIEPSAK RQKK         344