SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRPC25
Systematic nameYKL144C
DescriptionDNA-directed RNA polymerase III subunit RPC8

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2   HECTNARSP5   
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidenceUb-conjugate Confirmation
Methodprotein microarray (in vitro)
Reference"Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast."
Gupta R, Kus B, Fladd C, Wasmuth J, Tonikian R, Sidhu S, Krogan NJ, Parkinson J, Rotin D.
Mol Syst Biol. 3:116 (2007) [17551511]

Cross References
Swiss-Prot accessionP35718
SGD YKL144C
Gene Ontology (GO)
Cellular componentGO:0005666DNA-directed RNA polymerase III complexTAS:SGD.
Molecular functionGO:0003899DNA-directed RNA polymerase activityTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
162Phosphoserine.

Sequence Information
Sequence length212
Molecular weight24326

	---------+----------+----------+----------+----------+
MFILSKIADL VRIPPDQFHR DTISAITHQL NNKFANKIIP NVGLCITIYD   50
LLTVEEGQLK PGDGSSYINV TFRAVVFKPF LGEIVTGWIS KCTAEGIKVS   100
LLGIFDDIFI PQNMLFEGCY YTPEESAWIW PMDEETKLYF DVNEKIRFRI   150
EREVFVDVKP KSPKERELEE RAQLENEIEG KNEETPQNEK PPAYALLGSC   200
QTDGMGLVSW WE                                            212