SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRPB7
Systematic nameYDR404C
DescriptionDNA-directed RNA polymerase II subunit RPB7

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MONO HECTNARSP5   
* Blank: not determined, NA: not applicable.

References
[1]EvidenceUb-conjugate Confirmation
Methodluminescent assay (biotin-ubiquitin, GST-substrate)
Reference"A high throughput screen to identify substrates for the ubiquitin ligase Rsp5."
Kus B, Gajadhar A, Stanger K, Cho R, Sun W, Rouleau N, Lee T, Chan D, Wolting C, Edwards A, Bosse R, Rotin D.
J Biol Chem. 280(33):29470-8 (2005) [15955809]

Cross References
Swiss-Prot accessionP34087
SGD YDR404C
Gene Ontology (GO)
Cellular componentGO:0005665DNA-directed RNA polymerase II, core complexTAS:SGD.
Molecular functionGO:0003899DNA-directed RNA polymerase activityTAS:SGD.
Biological processGO:0006366transcription from RNA polymerase II promoterTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length171
Molecular weight19058

	---------+----------+----------+----------+----------+
MFFIKDLSLN ITLHPSFFGP RMKQYLKTKL LEEVEGSCTG KFGYILCVLD   50
YDNIDIQRGR ILPTDGSAEF NVKYRAVVFK PFKGEVVDGT VVSCSQHGFE   100
VQVGPMKVFV TKHLMPQDLT FNAGSNPPSY QSSEDVITIK SRIRVKIEGC   150
ISQVSSIHAI GSIKEDYLGA I                                  171