SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRPA2
Systematic nameYPR010C
AliasRPA135, RRN2, SRP3
DescriptionDNA-directed RNA polymerase I subunit RPA2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]

Cross References
Swiss-Prot accessionP22138
SGD YPR010C
Gene Ontology (GO)
Cellular componentGO:0005736DNA-directed RNA polymerase I complexTAS:SGD.
Molecular functionGO:0003899DNA-directed RNA polymerase activityTAS:SGD.
EC number 2.7.7.6

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
81Phosphoserine.
88Phosphoserine.

Sequence Information
Sequence length1203
Molecular weight135742

	---------+----------+----------+----------+----------+
MSKVIKPPGQ ARTADFRTLE RESRFINPPK DKSAFPLLQE AVQPHIGSFN   50
ALTEGPDGGL LNLGVKDIGE KVIFDGKPLN SEDEISNSGY LGNKLSVSVE   100
QVSIAKPMSN DGVSSAVERK VYPSESRQRL TSYRGKLLLK LKWSVNNGEE   150
NLFEVRDCGG LPVMLQSNRC HLNKMSPYEL VQHKEESDEI GGYFIVNGIE   200
KLIRMLIVQR RNHPMAIIRP SFANRGASYS HYGIQIRSVR PDQTSQTNVL   250
HYLNDGQVTF RFSWRKNEYL VPVVMILKAL CHTSDREIFD GIIGNDVKDS   300
FLTDRLELLL RGFKKRYPHL QNRTQVLQYL GDKFRVVFQA SPDQSDLEVG   350
QEVLDRIVLV HLGKDGSQDK FRMLLFMIRK LYSLVAGECS PDNPDATQHQ   400
EVLLGGFLYG MILKEKIDEY LQNIIAQVRM DINRGMAINF KDKRYMSRVL   450
MRVNENIGSK MQYFLSTGNL VSQSGLDLQQ VSGYTVVAEK INFYRFISHF   500
RMVHRGSFFA QLKTTTVRKL LPESWGFLCP VHTPDGSPCG LLNHFAHKCR   550
ISTQQSDVSR IPSILYSLGV APASHTFAAG PSLCCVQIDG KIIGWVSHEQ   600
GKIIADTLRY WKVEGKTPGL PIDLEIGYVP PSTRGQYPGL YLFGGHSRML   650
RPVRYLPLDK EDIVGPFEQV YMNIAVTPQE IQNNVHTHVE FTPTNILSIL   700
ANLTPFSDFN QSPRNMYQCQ MGKQTMGTPG VALCHRSDNK LYRLQTGQTP   750
IVKANLYDDY GMDNFPNGFN AVVAVISYTG YDMDDAMIIN KSADERGFGY   800
GTMYKTEKVD LALNRNRGDP ITQHFGFGND EWPKEWLEKL DEDGLPYIGT   850
YVEEGDPICA YFDDTLNKTK IKTYHSSEPA YIEEVNLIGD ESNKFQELQT   900
VSIKYRIRRT PQIGDKFSSR HGQKGVCSRK WPTIDMPFSE TGIQPDIIIN   950
PHAFPSRMTI GMFVESLAGK AGALHGIAQD STPWIFNEDD TPADYFGEQL   1000
AKAGYNYHGN EPMYSGATGE ELRADIYVGV VYYQRLRHMV NDKFQVRSTG   1050
PVNSLTMQPV KGRKRHGGIR VGEMERDALI GHGTSFLLQD RLLNSSDYTQ   1100
ASVCRECGSI LTTQQSVPRI GSISTVCCRR CSMRFEDAKK LLTKSEDGEK   1150
IFIDDSQIWE DGQGNKFVGG NETTTVAIPF VLKYLDSELS AMGIRLRYNV   1200
EPK                                                      1203