SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameROM2
Systematic nameYLR371W
DescriptionRHO1 GDP-GTP exchange protein 2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP51862
SGD YLR371W
Gene Ontology (GO)
Cellular componentGO:0005934cellular bud tipIDA:SGD.
Molecular functionGO:0005089Rho guanyl-nucleotide exchange factor activityIDA:SGD.
Molecular functionGO:0004871signal transducer activityIPI:SGD.
Biological processGO:0007015actin filament organizationTAS:SGD.
Biological processGO:0007117budding cell bud growthIPI:SGD.
Biological processGO:0007047cell wall organization and biogenesisIPI:SGD.
Biological processGO:0006897endocytosisIMP:SGD.
Biological processGO:0030468establishment of cell polarity (sensu Fungi)IPI:SGD.
Biological processGO:0006950response to stressIMP:SGD.
Biological processGO:0007264small GTPase mediated signal transductionIPI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
76Phosphoserine.
193Phosphoserine.
223Phosphoserine.
385Phosphoserine.

Sequence Information
Sequence length1356
Molecular weight152596

	---------+----------+----------+----------+----------+
MSETNVDSLG DRNDIYSQIF GVERRPDSFA TFDSDSHGDI SSQLLPNRIE   50
NIQNLNVLLS EDIANDIIIA KQRRRSGVEA AIDDSDIPNN EMKGKSSNYI   100
LSQQTNIKEV PDTQSLSSAD NTPVSSPKKA RDATSSHPIV HAKSMSHIYS   150
TSNSASRQAK HYNDHPLPPM SPRNEVYQKN KSTTAFVPKR KPSLPQLALA   200
GLKKQSSFST GSASTTPTQA RKSPLQGFGF FSRPSSKDLH EQHQHHQHIQ   250
HNNINNHNNN NTNNNGAHYQ VGSSNSNYPQ HSHSISSRSM SLNSSTLKNI   300
ASSFQSKTSN SRKATQKYDI TSNPFSDPHH HHHHHHSSNS HSSLNNVHGS   350
GNSSSVMGSS SNIGLGLKTR VSSTSLALKR YTSVSGTSLS SPRRSSMTPL   400
SASRPVMSAS SKKPQVYPAL LSRVATKFKS SIQLGEHKKD GLVYRDAFTG   450
QQAVDVICAI IRTSDRNLAL LFGRSLDAQK LFHDVVYEHR LRDSPHEVYE   500
FTDNSRFTGT GSTNAHDPLM LLPNSSSFNS GNHSYPNSGM VPSSSTSSLN   550
SDQATLTGSR LHMSSSLSQQ KNPAAIHNVN GVFTLLAECY SPTCTRDALC   600
YSISCPRRLE QQARLNLKPN GGLKRNISMA LDDDDEEKPS WTSSVSKEDW   650
ENLPKKEIKR QEAIYEVYIT EKNFVKSLEI TRDTFMKTLA ETNIISADIR   700
KNFIKHVFAH INDIYSVNRR FLKALTDRQR SSPVVRGIGD IVLRFIPFFE   750
PFVSYVASRP YAKYLIETQR SVNPYFARFD DDMMSSSLRH GIDSFLSQGV   800
SRPGRYMLLV KEIMKSTDPE KDKSDYEDLS KAMDALRDFM KRIDQASGAA   850
QDRHDVKLLK QKILFKNEYV NLGLNDERRK IKHEGILSRK ELSKSDGTVV   900
GDIQFYLLDN MLLFLKAKAV NKWHQHKVFQ RPIPLPLLFA CPGEDMPALR   950
KYIGDHPDCS GTVIQPEYNT SNPKNAITFL YYGAKQRYQV TLYAAQYAGL   1000
QTLLEKIKQG QAAIISKTEM FNVTKMSDRF FDYTNKINSV TSCDGGRKLL   1050
IATNSGLYMS NIKRQQNKDH RHKSSAFFST PIQLVQRNNI TQIAVLEEFK   1100
SIILLIDKKL YSCPLSLIEA EGNGTSFFKK HHKELINHVS FFAEGDCNGK   1150
RLIVTAHSSS HSIKYFEHEH PLLAEKNGSG SGNKKSLKKK ITEVIFDSEP   1200
VSISFLKANL CIGCKKGFQI VSISQNAHES LLDPADTSLE FALRDTLKPM   1250
AIYRVGNMFL LCYTEFAFFV NNQGWRKKES HIIHWEGEPQ KFAIWYPYIL   1300
AFDSNFIEIR KIETGELIRC VLADKIRLLQ TSTQEILYCY EDYRGYDTVA   1350
SLDFWG                                                   1356