SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRIO2
Systematic nameYNL207W
DescriptionSerine/threonine-protein kinase RIO2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP40160
SGD YNL207W
Gene Ontology (GO)
Cellular componentGO:0005829cytosolIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0005487nucleocytoplasmic transporter activityIGI:SGD.
Molecular functionGO:0004672protein kinase activityIDA:SGD.
Biological processGO:0030490processing of 20S pre-rRNAIMP:SGD.
EC number 2.7.11.1

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length425
Molecular weight49125

	---------+----------+----------+----------+----------+
MKLDTSHMRY LTTDDFRVLQ AVEQGSRSHE VVPTPLIHQI SGMRSQSGTN   50
RAISDLAKLS LISKMRNVKY DGYRLTYNGI DYLALKTMLN RDTVYSVGNT   100
IGVGKESDIY KVSDKNGNPR VMKIHRLGRT SFHSVRNNRD YLKKSNQGAN   150
WMHLSRLAAN KEYQFMSMLY SKGFKVPEPF DNSRHIVVME LIEGYPMRRL   200
RKHKNIPKLY SDLMCFIVDL ANSGLIHCDF NEFNIMIKDK LEDENDCGFV   250
VIDFPQCISI QHQDADYYFQ RDVDCIRRFF KKKLKYEPKP DSSMLDTEGF   300
GDGYKYAYPD FKRDVKRTDN LDELVQASGF SKKHPGDRGL ETAVESMRNA   350
VYNSDDDMSN DEAEEENGEG DYSEEDEYYD SELDNESSED DSEDAQEEEN   400
ERIIEALSSG VENLKMDKLG NYILE                              425