SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRIM101
Systematic nameYHL027W
AliasRIM1
DescriptionpH-response transcription factor pacC/RIM101

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP33400
SGD YHL027W
Gene Ontology (GO)
Cellular componentGO:0005634nucleusIC:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0016566specific transcriptional repressor activityIDA:SGD.
Biological processGO:0009272chitin- and beta-glucan-containing cell wal...IGI:SGD.
Biological processGO:0007126meiosisIMP:SGD.
Biological processGO:0000122negative regulation of transcription from R...IDA:SGD.
Biological processGO:0009268response to pHIMP:SGD.
Biological processGO:0030437sporulation (sensu Fungi)IMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length625
Molecular weight68232

	---------+----------+----------+----------+----------+
MVPLEDLLNK ENGTAAPQHS RESIVENGTD VSNVTKKDGL PSPNLSKRSS   50
DCSKRPRIRC TTEAIGLNGQ EDERMSPGST SSSCLPYHST SHLNTPPYDL   100
LGASAVSPTT SSSSDSSSSS PLAQAHNPAG DDDDADNDGD SEDITLYCKW   150
DNCGMIFNQP ELLYNHLCHD HVGRKSHKNL QLNCHWGDCT TKTEKRDHIT   200
SHLRVHVPLK PFGCSTCSKK FKRPQDLKKH LKIHLESGGI LKRKRGPKWG   250
SKRTSKKNKS CASDAVSSCS ASVPSAIAGS FKSHSTSPQI LPPLPVGISQ   300
HLPSQQQQRA ISLNQLCSDE LSQYKPVYSP QLSARLQTIL PPLYYNNGST   350
VSQGANSRSM NVYEDGCSNK TIANATQFFT KLSRNMTNNY ILQQSGGSTE   400
SSSSSGRIPV AQTSYVQPPN APSYQSVQGG SSISATANTA TYVPVRLAKY   450
PTGPSLTEHL PPLHSNTAGG VFNRQSQYAM PHYPSVRAAP SYSSSGCSIL   500
PPLQSKIPML PSRRTMAGGT SLKPNWEFSL NQKSCTNDII MSKLAIEEVD   550
DESEIEDDFV EMLGIVNIIK DYLLCCVMED LDDEESEDKD EENAFLQESL   600
EKLSLQNQMG TNSVRILTKY PKILV                              625