SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRFA1
Systematic nameYAR007C
AliasBUF2, RPA1
DescriptionReplication factor A protein 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]

Cross References
Swiss-Prot accessionP22336
SGD YAR007C
Gene Ontology (GO)
Cellular componentGO:0000781chromosome, telomeric regionIMP:SGD.
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005662DNA replication factor A complexTAS:SGD.
Molecular functionGO:0003684damaged DNA bindingTAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0003697single-stranded DNA bindingTAS:SGD.
Biological processGO:0006269DNA replication, synthesis of RNA primerTAS:SGD.
Biological processGO:0006271DNA strand elongation during DNA replicationTAS:SGD.
Biological processGO:0006268DNA unwinding during replicationTAS:SGD.
Biological processGO:0000724double-strand break repair via homologous r...IGI:SGD.
Biological processGO:0006289nucleotide-excision repairTAS:SGD.
Biological processGO:0006301postreplication repairTAS:SGD.
Biological processGO:0000722telomere maintenance via recombinationIGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
178Phosphoserine, by ATM or ATR.

Sequence Information
Sequence length621
Molecular weight70348

	---------+----------+----------+----------+----------+
MSSVQLSRGD FHSIFTNKQR YDNPTGGVYQ VYNTRKSDGA NSNRKNLIMI   50
SDGIYHMKAL LRNQAASKFQ SMELQRGDII RVIIAEPAIV RERKKYVLLV   100
DDFELVQSRA DMVNQTSTFL DNYFSEHPNE TLKDEDITDS GNVANQTNAS   150
NAGVPDMLHS NSNLNANERK FANENPNSQK TRPIFAIEQL SPYQNVWTIK   200
ARVSYKGEIK TWHNQRGDGK LFNVNFLDTS GEIRATAFND FATKFNEILQ   250
EGKVYYVSKA KLQPAKPQFT NLTHPYELNL DRDTVIEECF DESNVPKTHF   300
NFIKLDAIQN QEVNSNVDVL GIIQTINPHF ELTSRAGKKF DRRDITIVDD   350
SGFSISVGLW NQQALDFNLP EGSVAAIKGV RVTDFGGKSL SMGFSSTLIP   400
NPEIPEAYAL KGWYDSKGRN ANFITLKQEP GMGGQSAASL TKFIAQRITI   450
ARAQAENLGR SEKGDFFSVK AAISFLKVDN FAYPACSNEN CNKKVLEQPD   500
GTWRCEKCDT NNARPNWRYI LTISIIDETN QLWLTLFDDQ AKQLLGVDAN   550
TLMSLKEEDP NEFTKITQSI QMNEYDFRIR AREDTYNDQS RIRYTVANLH   600
SLNYRAEADY LADELSKALL A                                  621