SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRET1
Systematic nameYDL145C
AliasCOP1, SEC33
DescriptionCoatomer subunit alpha

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP53622
SGD YDL145C
Gene Ontology (GO)
Cellular componentGO:0030126COPI vesicle coatIMP:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006888ER to Golgi vesicle-mediated transportIMP:SGD.
Biological processGO:0006890retrograde vesicle-mediated transport, Golg...IMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length1201
Molecular weight135607

	---------+----------+----------+----------+----------+
MKMLTKFESK STRAKGIAFH PSRPWVLVAL FSSTIQLWDY RMGTLLHRFE   50
DHEGPVRGLD FHPTQPIFVS AGDDYTIKVW SLDTNKCLYT LTGHLDYVRT   100
VFFHRELPWI ISASDDQTIR IWNWQNRKEI ACLTGHNHFV MCAQFHPTDD   150
LIVSASLDET IRIWDISGLR KRHSAPGTSS FEEQMSAQQN LLDGSLGDCV   200
VKFILEGHTR GVNWASFHPT LPLIVSGSDD RQVKLWRMSA TKAWEVDTCR   250
GHTNNVDSVI FHPHQNLIIS VGEDKTLRVW DLDKRTPVKQ FKRENDRFWL   300
IAAHPHINLF GAAHDSGIMV FKLDRERPCS FIHQNQLFFV NAEKQIQSFN   350
FQKRVASLPY ASLKGIGQPW DAFRSISYNP SQHSVLVNEA NGKFALVILP   400
KQPVGAVEPT SVTQDTGNFA TFVGRNRFVV YNKNTESVEV RSLENKVTRN   450
IKVEETVRTI VAAGPGSVLV IHPREVILYD VQQGKKVSQL AVKNVKYVSW   500
SLDGQYVALM SKHTITLATK KLELINSMHE TIRIKSAAWD ETGVLIYSTL   550
NHIRYSLLNG DRGIIKTLEK TLYITKVQGK LVYCLNREGE IEILTIDPTE   600
YRFKKALVNK NFPEVLRLIK DSNLVGQNII SYLQKSGYPE IALQFVQDPH   650
IRFDLALEYG NLDVALDEAK KLNDSSTWER LIQEALAQGN ASLAEMIYQT   700
QHSFDKLSFL YLVTGDVNKL SKMQNIAQTR EDFGSMLLNT FYNNSTKERS   750
SIFAEGGSLP LAYAVAKANG DEAAASAFLE QAEVDEQDVT LPDQMDASNF   800
VQRPVISKPL EKWPLKEAEL SYFEKAVLGQ IDDLTIDDET PAVNTTQEQE   850
EPLGEENFND EDIGEDEGAW DLGDEDLDVG EELPEEVEQG EITSPAQEVE   900
TAIWIKNSKL PAVLVAAGAF DAAVQALSKQ VGVVKLEPLK KYFTNIYEGC   950
RTYIPSTPCE LPAQLGYVRA YDDTVSEDQI LPYVPGLDVV NEKMNEGYKN   1000
FKLNKPDIAI ECFREAIYRI TLLMVDDAED EKLAHKILET AREYILGLSI   1050
ELERRSLKEG NTVRMLELAA YFTKAKLSPI HRTNALQVAM SQHFKHKNFL   1100
QASYFAGEFL KIISSGPRAE QARKIKNKAD SMASDAIPID FDPYAKFDIC   1150
AATYKPIYED TPSVSDPLTG SKYVITEKDK IDRIAMISKI GAPASGLRIR   1200
V                                                        1201