SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameREB1
Systematic nameYBR049C
AliasGRF2
DescriptionDNA-binding protein REB1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]

Cross References
Swiss-Prot accessionP21538
SGD YBR049C
Gene Ontology (GO)
Cellular componentGO:0005634nucleusTAS:SGD.
Molecular functionGO:0003716RNA polymerase I transcription termination ...TAS:SGD.
Molecular functionGO:0003702RNA polymerase II transcription factor acti...IDA:SGD.
Biological processGO:0006357regulation of transcription from RNA polyme...TAS:SGD.
Biological processGO:0006363transcription termination from RNA polymera...TAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
355Phosphoserine.
357Phosphoserine.

Sequence Information
Sequence length810
Molecular weight91874

	---------+----------+----------+----------+----------+
MPSGHNDKNA NQESVEEAVL KYVGVGLDHQ NHDPQLHTKD LENKHSKKQN   50
IVESSSDVDV NNNDDSNRNE DNNDDSENIS ALNANESSSN VDHANSNEQH   100
NAVMDWYLRQ TAHNQQDDED DENNNNTDNG NDSNNHFSQS DIVVDDDDDK   150
NKKDAGVGVD DDHQSMAMAA VAAAYTLSKN NNNNNSIAND SNSRKRQHDN   200
GNNHENSQKK RKNNNDDDDR QIGNVDPELT TLGDADDNDT NNDVIDRDQL   250
VHKAIIDADS ITQHPDFQQY LNTAADTDDN EKLKHIKDHL MRTHGLNHQN   300
KNHNDDTDDL SNSTKQYSEL QKDSMLDSSL NKSRNYMEVL PKVISQDTQP   350
HQQKSPSHDN EAGSVDNSEI SQLLQSAATK ASSLVSLSSS SATPSTSRSN   400
NSKAFDKAED AALERFINEY EAIERLTRQQ VCERIWSSDR PKDNFWNNIY   450
KVLPYRSSSS IYKHMRRKYH IFEQRGKWTA EEEQELAKLC AEKEGQWAEI   500
GKTLGRMPED CRDRWRNYVK CGTNRASNRW SVEEEELLKK VISDMLEEAQ   550
QQQSQLHPNL LEEEQHLLQD DQNDHRNNDE DDDDTASAAA AAAAAIQEQQ   600
QLLQQKQQDD DDAIAAAAAA ASSSLGDNKD EDKPHDSLGI QLDDNSQNSM   650
VPAPSATSTH SKSLSNTIRR HNNKLRKSLM GNGKLDFKDI INWTIVSERM   700
GGTRSRIQCR YKWNKLVKRE AIAKIQTVKD DDMLWIFEKL RDLGITEDSQ   750
VDWDELAALK PGMKLNGLEL KLCYERMKKK VKGYKQKSIN EISKELVDYF   800
SSNISMKTEN                                                810