SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRCR2
Systematic nameYDR003W
AliasSSH5
DescriptionProtein RCR2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1   HECTNARSP5   
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidenceUb-conjugate Confirmation
Methodprotein microarray (in vitro)
Reference"Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast."
Gupta R, Kus B, Fladd C, Wasmuth J, Tonikian R, Sidhu S, Krogan NJ, Parkinson J, Rotin D.
Mol Syst Biol. 3:116 (2007) [17551511]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ03446
SGD YDR003W
Gene Ontology (GO)
Cellular componentGO:0000324vacuole, cell cycle-correlated morphologyIDA:SGD.
Cellular componentGO:0031982vesicleIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0016192vesicle-mediated transportIGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
161Phosphoserine.
189Phosphoserine.

Sequence Information
Sequence length210
Molecular weight24211

	---------+----------+----------+----------+----------+
MILREQIDFL IHKRQDDNNN NGEAITDDDP FSSSSWRWGR WIFFIFFIVA   50
LLILLFSTAK VNRRRRIMGQ APIRGTAWLT PPTYRQSERD YNGTQRCVED   100
YVPEYTETAN ENDLGFYDER GEFHPNGKTE YLAPPPLSEE QASSTDKDLQ   150
RPVAAVVRIP SESEFDFNLL RPTMNNFVNG QSNRNEQHSP TVESSSFDVN   200
NAPARAKVSK                                                210