SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRAD6
Systematic nameYGL058W
AliasUBC2
DescriptionUbiquitin-conjugating enzyme E2 2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP06104
SGD YGL058W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Cellular componentGO:0000502proteasome complex (sensu Eukaryota)IPI:SGD.
Molecular functionGO:0004842ubiquitin-protein ligase activityIDA:SGD.
Biological processGO:0016574histone ubiquitinationIDA:SGD.
Biological processGO:0006301postreplication repairIMP:SGD.
Biological processGO:0006513protein monoubiquitinationIMP:SGD.
Biological processGO:0000723telomere maintenanceIMP:SGD.
Biological processGO:0006511ubiquitin-dependent protein catabolic processIMP:SGD.
EC number 6.3.2.19

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
120Phosphoserine, by SGV1.

Sequence Information
Sequence length172
Molecular weight19706

	---------+----------+----------+----------+----------+
MSTPARRRLM RDFKRMKEDA PPGVSASPLP DNVMVWNAMI IGPADTPYED   50
GTFRLLLEFD EEYPNKPPHV KFLSEMFHPN VYANGEICLD ILQNRWTPTY   100
DVASILTSIQ SLFNDPNPAS PANVEAATLF KDHKSQYVKR VKETVEKSWE   150
DDMDDMDDDD DDDDDDDDDE AD                                 172