SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard nameRAD53
Systematic nameYPL153C
AliasMEC2, SAD1, SPK1
DescriptionSerine/threonine-protein kinase RAD53

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP22216
SGD YPL153C
Gene Ontology (GO)
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0003688DNA replication origin bindingIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0004712protein threonine/tyrosine kinase activityIDA:SGD.
Biological processGO:0009202deoxyribonucleoside triphosphate biosynthet...IMP:SGD.
Biological processGO:0006281DNA repairIMP:SGD.
Biological processGO:0006270DNA replication initiationIMP:SGD.
EC number 2.7.11.1

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
165Phosphoserine.
424Phosphoserine.
560Phosphoserine.
774Phosphoserine.
789Phosphoserine.
791Phosphoserine.
793Phosphoserine.

Sequence Information
Sequence length821
Molecular weight91962

	---------+----------+----------+----------+----------+
MENITQPTQQ STQATQRFLI EKFSQEQIGE NIVCRVICTT GQIPIRDLSA   50
DISQVLKEKR SIKKVWTFGR NPACDYHLGN ISRLSNKHFQ ILLGEDGNLL   100
LNDISTNGTW LNGQKVEKNS NQLLSQGDEI TVGVGVESDI LSLVIFINDK   150
FKQCLEQNKV DRIRSNLKNT SKIASPGLTS STASSMVANK TGIFKDFSII   200
DEVVGQGAFA TVKKAIERTT GKTFAVKIIS KRKVIGNMDG VTRELEVLQK   250
LNHPRIVRLK GFYEDTESYY MVMEFVSGGD LMDFVAAHGA VGEDAGREIS   300
RQILTAIKYI HSMGISHRDL KPDNILIEQD DPVLVKITDF GLAKVQGNGS   350
FMKTFCGTLA YVAPEVIRGK DTSVSPDEYE ERNEYSSLVD MWSMGCLVYV   400
ILTGHLPFSG STQDQLYKQI GRGSYHEGPL KDFRISEEAR DFIDSLLQVD   450
PNNRSTAAKA LNHPWIKMSP LGSQSYGDFS QISLSQSLSQ QKLLENMDDA   500
QYEFVKAQRK LQMEQQLQEQ DQEDQDGKIQ GFKIPAHAPI RYTQPKSIEA   550
ETREQKLLHS NNTENVKSSK KKGNGRFLTL KPLPDSIIQE SLEIQQGVNP   600
FFIGRSEDCN CKIEDNRLSR VHCFIFKKRH AVGKSMYESP AQGLDDIWYC   650
HTGTNVSYLN NNRMIQGTKF LLQDGDEIKI IWDKNNKFVI GFKVEINDTT   700
GLFNEGLGML QEQRVVLKQT AEEKDLVKKL TQMMAAQRAN QPSASSSSMS   750
AKKPPVSDTN NNGNNSVLND LVESPINANT GNILKRIHSV SLSQSQIDPS   800
KKVKRAKLDQ TSKGPENLQF S                                  821