SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard nameRAD52
Systematic nameYML032C
DescriptionDNA repair and recombination protein RAD52

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP06778
SGD YML032C
Gene Ontology (GO)
Cellular componentGO:0000228nuclear chromosomeIDA:SGD.
Molecular functionGO:0000739DNA strand annealing activityIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0000150recombinase activityIDA:SGD.
Biological processGO:0000727double-strand break repair via break-induce...TAS:SGD.
Biological processGO:0045002double-strand break repair via single-stran...IGI:SGD.
Biological processGO:0000707meiotic DNA recombinase assemblyTAS:SGD.
Biological processGO:0006301postreplication repairIMP:SGD.
Biological processGO:0000722telomere maintenance via recombinationIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length471
Molecular weight52404

	---------+----------+----------+----------+----------+
MNEIMDMDEK KPVFGNHSED IQTKLDKKLG PEYISKRVGF GTSRIAYIEG   50
WRVINLANQI FGYNGWSTEV KSVVIDFLDE RQGKFSIGCT AIVRVTLTSG   100
TYREDIGYGT VENERRKPAA FERAKKSAVT DALKRSLRGF GNALGNCLYD   150
KDFLAKIDKV KFDPPDFDEN NLFRPTDEIS ESSRTNTLHE NQEQQQYPNK   200
RRQLTKVTNT NPDSTKNLVK IENTVSRGTP MMAAPAEANS KNSSNKDTDL   250
KSLDASKQDQ DDLLDDSLMF SDDFQDDDLI NMGNTNSNVL TTEKDPVVAK   300
QSPTASSNPE AEQITFVTAK AATSVQNERY IGEESIFDPK YQAQSIRHTV   350
DQTTSKHIPA SVLKDKTMTT ARDSVYEKFA PKGKQLSMKN NDKELGPHML   400
EGAGNQVPRE TTPIKTNATA FPPAAAPRFA PPSKVVHPNG NGAVPAVPQQ   450
RSTRREVGRP KINPLHARKP T                                  471