SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRAD4
Systematic nameYER162C
DescriptionDNA repair protein RAD4

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1   HECTNAUFD4   
2   RINGECSRAD7  Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidenceProtein Stability Assay
MethodWestern blot
Reference"A synthetic defect in protein degradation caused by loss of Ufd4 and Rad23."
Ju D, Xie Y.
Biochem Biophys Res Commun. 341(2):648-52 (2006) [16430867]
[2]EvidenceUb-conjugate Confirmation
MethodWestern blot (in vitro)
Reference"Distinct functions of the ubiquitin-proteasome pathway influence nucleotide excision repair."
Gillette TG, Yu S, Zhou Z, Waters R, Johnston SA, Reed SH.
EMBO J. 25(11):2529-38 (2006) [16675952]

Cross References
Swiss-Prot accessionP14736
SGD YER162C
Gene Ontology (GO)
Cellular componentGO:0000111nucleotide-excision repair factor 2 complexIDA:SGD.
Cellular componentGO:0000108repairosomeIDA:SGD.
Molecular functionGO:0003684damaged DNA bindingIDA:SGD.
Biological processGO:0000715nucleotide-excision repair, DNA damage reco...TAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length754
Molecular weight87174

	---------+----------+----------+----------+----------+
MNEDLPKEYF ELIRKALNEK EAEKAPLSRR RRVRRKNQPL PDAKKKFKTG   50
LNELPRESVV TVNLDSSDDG VVTVPTDDSV EEIQSSEEDY DSEEFEDVTD   100
GNEVAGVEDI SVEIKPSSKR NSDARRTSRN VCSNEERKRR KYFHMLYLVC   150
LMVHGFIRNE WINSKRLSRK LSNLVPEKVF ELLHPQKDEE LPLRSTRKLL   200
DGLKKCMELW QKHWKITKKY DNVGIYMRTW KEIEMSANNK RKFKTLKRSD   250
FLRAVSKGHG DPDISVQGFV AMLRACNVNA RLIMSCQPPD FTNMKIDTSL   300
NGNNAYKDMV KYPIFWCEVW DKFSKKWITV DPVNLKTIEQ VRLHSKLAPK   350
GVACCERNML RYVIAYDRKY GCRDVTRRYA QWMNSKVRKR RITKDDFGEK   400
WFRKVITALH HRKRTKIDDY EDQYFFQRDE SEGIPDSVQD LKNHPYYVLE   450
QDIKQTQIVK PGCKECGYLK VHGKVGKVLK VYAKRDIADL KSARQWYMNG   500
RILKTGSRCK KVIKRTVGRP KGEAEEEDER LYSFEDTELY IPPLASASGE   550
ITKNTFGNIE VFAPTMIPGN CCLVENPVAI KAARFLGVEF APAVTSFKFE   600
RGSTVKPVLS GIVVAKWLRE AIETAIDGIE FIQEDDNRKE HLLGALESWN   650
TLLLKLRIRS KLNSTYGKIA EEEPNVTKEQ NIADNHDNTE TFMGGGFLPG   700
IANHEARPYS EPSEPEDSLD YVSVDKAEES ATDDDVGEDY SDFMKELEMS   750
EESD                                                     754