SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRAD30
Systematic nameYDR419W
AliasDBH1
DescriptionDNA polymerase eta

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI RINGSCFUFO1  Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidenceProtein Stability Assay
MethodWestern blot
Reference"Polymerase eta is a short-lived, proteasomally degraded protein that is temporarily stabilized following UV irradiation in Saccharomyces cerevisiae."
Skoneczna A, McIntyre J, Skoneczny M, Policinska Z, Sledziewska-Gojska E.
J Mol Biol. 366(4):1074-86 (2007) [17198712]

Cross References
Swiss-Prot accessionQ04049
SGD YDR419W
Gene Ontology (GO)
Cellular componentGO:0005657replication forkIDA:SGD.
Molecular functionGO:0015999eta DNA polymerase activityIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0019985bypass DNA synthesisIGI:SGD.
Biological processGO:0006260DNA replicationIDA:SGD.
Biological processGO:0006280mutagenesisIGI:SGD.
EC number 2.7.7.7

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length632
Molecular weight71515

	---------+----------+----------+----------+----------+
MSKFTWKELI QLGSPSKAYE SSLACIAHID MNAFFAQVEQ MRCGLSKEDP   50
VVCVQWNSII AVSYAARKYG ISRMDTIQEA LKKCSNLIPI HTAVFKKGED   100
FWQYHDGCGS WVQDPAKQIS VEDHKVSLEP YRRESRKALK IFKSACDLVE   150
RASIDEVFLD LGRICFNMLM FDNEYELTGD LKLKDALSNI REAFIGGNYD   200
INSHLPLIPE KIKSLKFEGD VFNPEGRDLI TDWDDVILAL GSQVCKGIRD   250
SIKDILGYTT SCGLSSTKNV CKLASNYKKP DAQTIVKNDC LLDFLDCGKF   300
EITSFWTLGG VLGKELIDVL DLPHENSIKH IRETWPDNAG QLKEFLDAKV   350
KQSDYDRSTS NIDPLKTADL AEKLFKLSRG RYGLPLSSRP VVKSMMSNKN   400
LRGKSCNSIV DCISWLEVFC AELTSRIQDL EQEYNKIVIP RTVSISLKTK   450
SYEVYRKSGP VAYKGINFQS HELLKVGIKF VTDLDIKGKN KSYYPLTKLS   500
MTITNFDIID LQKTVVDMFG NQVHTFKSSA GKEDEEKTTS SKADEKTPKL   550
ECCKYQVTFT DQKALQEHAD YHLALKLSEG LNGAEESSKN LSFGEKRLLF   600
SRKRPNSQHT ATPQKKQVTS SKNILSFFTR KK                      632