SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRAD23
Systematic nameYEL037C
DescriptionUV excision repair protein RAD23

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP32628
SGD YEL037C
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Cellular componentGO:0000111nucleotide-excision repair factor 2 complexIDA:SGD.
Cellular componentGO:0000502proteasome complex (sensu Eukaryota)IMP:SGD.
Cellular componentGO:0000108repairosomeIDA:SGD.
Molecular functionGO:0003684damaged DNA bindingIDA:SGD.
Molecular functionGO:0030674protein binding, bridgingIPI:SGD.
Biological processGO:0030433ER-associated protein catabolic processIMP:SGD.
Biological processGO:0042177negative regulation of protein catabolic pr...TAS:SGD.
Biological processGO:0000715nucleotide-excision repair, DNA damage reco...TAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
139Phosphothreonine.

Sequence Information
Sequence length398
Molecular weight42367

	---------+----------+----------+----------+----------+
MVSLTFKNFK KEKVPLDLEP SNTILETKTK LAQSISCEES QIKLIYSGKV   50
LQDSKTVSEC GLKDGDQVVF MVSQKKSTKT KVTEPPIAPE SATTPGRENS   100
TEASPSTDAS AAPAATAPEG SQPQEEQTAT TERTESASTP GFVVGTERNE   150
TIERIMEMGY QREEVERALR AAFNNPDRAV EYLLMGIPEN LRQPEPQQQT   200
AAAAEQPSTA ATTAEQPAED DLFAQAAQGG NASSGALGTT GGATDAAQGG   250
PPGSIGLTVE DLLSLRQVVS GNPEALAPLL ENISARYPQL REHIMANPEV   300
FVSMLLEAVG DNMQDVMEGA DDMVEGEDIE VTGEAAAAGL GQGEGEGSFQ   350
VDYTPEDDQA ISRLCELGFE RDLVIQVYFA CDKNEEAAAN ILFSDHAD     398