SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRAD16
Systematic nameYBR114W
DescriptionDNA repair protein RAD16

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP31244
SGD YBR114W
Gene Ontology (GO)
Cellular componentGO:0031463Cul3-RING ubiquitin ligase complexIDA:SGD.
Cellular componentGO:0000113nucleotide-excision repair factor 4 complexIDA:SGD.
Cellular componentGO:0000108repairosomeIDA:SGD.
Biological processGO:0000715nucleotide-excision repair, DNA damage reco...IDA:SGD.
Biological processGO:0042787protein ubiquitination during ubiquitin-dep...IMP:SGD.
EC number 3.6.1.-

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
25Phosphoserine.
49Phosphoserine.
109Phosphoserine.

Sequence Information
Sequence length790
Molecular weight91430

	---------+----------+----------+----------+----------+
MQEGGFIRRR RTRSTKKSVN YNELSDDDTA VKNSKTLQLK GNSENVNDSQ   50
DEEYRDDATL VKSPDDDDKD FIIDLTGSDK ERTATDENTH AIKNDNDEII   100
EIKEERDVSD DDEPLTKKRK TTARKKKKKT STKKKSPKVT PYERNTLRLY   150
EHHPELRNVF TDLKNAPPYV PQRSKQPDGM TIKLLPFQLE GLHWLISQEE   200
SIYAGGVLAD EMGMGKTIQT IALLMNDLTK SPSLVVAPTV ALMQWKNEIE   250
QHTKGQLKIY IYHGASRTTD IKDLQGYDVV LTTYAVLESV FRKQNYGFRR   300
KNGLFKQPSV LHNIDFYRVI LDEAHNIKDR QSNTARAVNN LKTQKRWCLS   350
GTPLQNRIGE MYSLIRFLNI NPFTKYFCTK CDCASKDWKF TDRMHCDHCS   400
HVIMQHTNFF NHFMLKNIQK FGVEGPGLES FNNIQTLLKN IMLRRTKVER   450
ADDLGLPPRI VTVRRDFFNE EEKDLYRSLY TDSKRKYNSF VEEGVVLNNY   500
ANIFTLITRM RQLADHPDLV LKRLNNFPGD DIGVVICQLC NDEAEEPIES   550
KCHHKFCRLC IKEYVESFME NNNKLTCPVC HIGLSIDLSQ PALEVDLDSF   600
KKQSIVSRLN MSGKWQSSTK IEALVEELYK LRSNKRTIKS IVFSQFTSML   650
DLVEWRLKRA GFQTVKLQGS MSPTQRDETI KYFMNNIQCE VFLVSLKAGG   700
VALNLCEASQ VFILDPWWNP SVEWQSGDRV HRIGQYRPVK ITRFCIEDSI   750
EARIIELQEK KANMIHATIN QDEAAISRLT PADLQFLFNN               790