SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePYK1
Systematic nameYAL038W
AliasCDC19
DescriptionPyruvate kinase 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP00549
SGD YAL038W
Gene Ontology (GO)
Molecular functionGO:0004743pyruvate kinase activityIDA:SGD.
Biological processGO:0006096glycolysisIMP:SGD.
Biological processGO:0006090pyruvate metabolic processIMP:SGD.
EC number 2.7.1.40

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
8Phosphothreonine.
9Phosphoserine.
21Phosphothreonine.
22Phosphoserine.
53Phosphoserine.
56Phosphoserine.
213Phosphoserine.
372Phosphothreonine.
402Phosphoserine.
478Phosphothreonine.
498Phosphoserine.

Sequence Information
Sequence length500
Molecular weight54545

	---------+----------+----------+----------+----------+
MSRLERLTSL NVVAGSDLRR TSIIGTIGPK TNNPETLVAL RKAGLNIVRM   50
NFSHGSYEYH KSVIDNARKS EELYPGRPLA IALDTKGPEI RTGTTTNDVD   100
YPIPPNHEMI FTTDDKYAKA CDDKIMYVDY KNITKVISAG RIIYVDDGVL   150
SFQVLEVVDD KTLKVKALNA GKICSHKGVN LPGTDVDLPA LSEKDKEDLR   200
FGVKNGVHMV FASFIRTAND VLTIREVLGE QGKDVKIIVK IENQQGVNNF   250
DEILKVTDGV MVARGDLGIE IPAPEVLAVQ KKLIAKSNLA GKPVICATQM   300
LESMTYNPRP TRAEVSDVGN AILDGADCVM LSGETAKGNY PINAVTTMAE   350
TAVIAEQAIA YLPNYDDMRN CTPKPTSTTE TVAASAVAAV FEQKAKAIIV   400
LSTSGTTPRL VSKYRPNCPI ILVTRCPRAA RFSHLYRGVF PFVFEKEPVS   450
DWTDDVEARI NFGIEKAKEF GILKKGDTYV SIQGFKAGAG HSNTLQVSTV   500