SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePUT4
Systematic nameYOR348C
DescriptionProline-specific permease

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1   HECTNARSP5 DOA4 
* Blank: not determined, NA: not applicable.

References
[1]EvidenceProtein Stability Assay
MethodWestern blot
Reference"Engineering of yeast Put4 permease and its application to lager yeast for efficient proline assimilation."
Omura F, Fujita A, Miyajima K, Fukui N.
Biosci Biotechnol Biochem. 69(6):1162-71 (2005) [15973048]

Cross References
Swiss-Prot accessionP15380
SGD YOR348C
Gene Ontology (GO)
Molecular functionGO:0005296L-proline permease activityIMP:SGD.
Biological processGO:0015804neutral amino acid transportIDA:SGD.
Biological processGO:0006562proline catabolic processTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length627
Molecular weight68788

	---------+----------+----------+----------+----------+
MVNILPFHKN NRHSAGVVTC ADDVSGDGSG GDTKKEEDVV QVTESPSSGS   50
RNNHRSDNEK DDAIRMEKIS KNQSASSNGT IREDLIMDVD LEKSPSVDGD   100
SEPHKLKQGL QSRHVQLIAL GGAIGTGLLV GTSSTLHTCG PAGLFISYII   150
ISAVIYPIMC ALGEMVCFLP GDGSDSAGST ANLVTRYVDP SLGFATGWNY   200
FYCYVILVAA ECTAASGVVE YWTTAVPKGV WITIFLCVVV ILNFSAVKVY   250
GESEFWFASI KILCIVGLII LSFILFWGGG PNHDRLGFRY WQHPGAFAHH   300
LTGGSLGNFT DIYTGIIKGA FAFILGPELV CMTSAECADQ RRNIAKASRR   350
FVWRLIFFYV LGTLAISVIV PYNDPTLVNA LAQGKPGAGS SPFVIGIQNA   400
GIKVLPHIIN GCILTSAWSA ANAFMFASTR SLLTMAQTGQ APKCLGRINK   450
WGVPYVAVGV SFLCSCLAYL NVSSSTADVF NWFSNISTIS GFLGWMCGCI   500
AYLRFRKAIF YNGLYDRLPF KTWGQPYTVW FSLIVIGIIT ITNGYAIFIP   550
KYWRVADFIA AYITLPIFLV LWFGHKLYTR TWRQWWLPVS EIDVTTGLVE   600
IEEKSREIEE MRLPPTGFKD KFLDALL                            627