SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePRS1
Systematic nameYKL181W
AliasPPS1, PRP1, PRPS, PRPS1
DescriptionRibose-phosphate pyrophosphokinase 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP32895
SGD YKL181W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0004749ribose phosphate diphosphokinase activityTAS:SGD.
Biological processGO:0006189'de novo' IMP biosynthetic processTAS:SGD.
Biological processGO:0006207'de novo' pyrimidine base biosynthetic processTAS:SGD.
Biological processGO:0000105histidine biosynthetic processTAS:SGD.
Biological processGO:0006166purine ribonucleoside salvageTAS:SGD.
Biological processGO:0000162tryptophan biosynthetic processTAS:SGD.
EC number 2.7.6.1

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
199Phosphoserine.
218Phosphoserine.

Sequence Information
Sequence length427
Molecular weight47047

	---------+----------+----------+----------+----------+
MRKCKIFVGN SHPELGNMVC QRLGIEPAPC TLKKFANGET SVQIGVSVRD   50
EDVYVIQSGS PSINDDIMEL LILVSACRGG SARKITAVIP QFPYSKQCKM   100
KRHRGAITAR MLANLLVMAG ADHVVSMDLH ASQMQGFFTK PVDNLYGGPS   150
LAKWIRENVE DYEDAVVVSK NPGGTKRVTA LADSLKINFA MIHTDRRRSK   200
DLYSQNKDLQ QLKLRKQSML RKNRPIIRQG DHPNEEENII LSNGIQTARI   250
RNGHVIGDDE ADDDEDAILE SDSELHSIDG LDSHGLGGTY DAVDSEDEEE   300
IPVLYREQLI TLVGNVRGRS AIILDDMIDR PGSFISAAEH LVQNCGAKKV   350
YVVATHGIFT GDCLEELEKS DAIDTIVVTN TYPISGERIA GSKKLVTIDV   400
SPIFAECIRR DHYGESISVL FDSLAAL                            427