SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePRE7
Systematic nameYBL041W
AliasPRS3, PTS1
DescriptionProteasome component C5

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP23724
SGD YBL041W
Gene Ontology (GO)
Cellular componentGO:0019774proteasome core complex, beta-subunit compl...IPI:SGD.
Molecular functionGO:0004175endopeptidase activityTAS:SGD.
Biological processGO:0006511ubiquitin-dependent protein catabolic processTAS:SGD.
EC number 3.4.25.1

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
209Phosphoserine.
211Phosphothreonine.

Sequence Information
Sequence length241
Molecular weight26871

	---------+----------+----------+----------+----------+
MATIASEYSS EASNTPIEHQ FNPYGDNGGT ILGIAGEDFA VLAGDTRNIT   50
DYSINSRYEP KVFDCGDNIV MSANGFAADG DALVKRFKNS VKWYHFDHND   100
KKLSINSAAR NIQHLLYGKR FFPYYVHTII AGLDEDGKGA VYSFDPVGSY   150
EREQCRAGGA AASLIMPFLD NQVNFKNQYE PGTNGKVKKP LKYLSVEEVI   200
KLVRDSFTSA TERHIQVGDG LEILIVTKDG VRKEFYELKR D            241