SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePRE2
Systematic nameYPR103W
AliasDOA3, PRG1
DescriptionProteasome component PRE2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP30656
SGD YPR103W
Gene Ontology (GO)
Cellular componentGO:0019774proteasome core complex, beta-subunit compl...IPI:SGD.
Molecular functionGO:0004175endopeptidase activityTAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006511ubiquitin-dependent protein catabolic processTAS:SGD.
EC number 3.4.25.1

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length287
Molecular weight31637

	---------+----------+----------+----------+----------+
MQAIADSFSV PNRLVKELQY DNEQNLESDF VTGASQFQRL APSLTVPPIA   50
SPQQFLRAHT DDSRNPDCKI KIAHGTTTLA FRFQGGIIVA VDSRATAGNW   100
VASQTVKKVI EINPFLLGTM AGGAADCQFW ETWLGSQCRL HELREKERIS   150
VAAASKILSN LVYQYKGAGL SMGTMICGYT RKEGPTIYYV DSDGTRLKGD   200
IFCVGSGQTF AYGVLDSNYK WDLSVEDALY LGKRSILAAA HRDAYSGGSV   250
NLYHVTEDGW IYHGNHDVGE LFWKVKEEEG SFNNVIG                 287