SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePRB1
Systematic nameYEL060C
DescriptionCerevisin

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP09232
SGD YEL060C
Gene Ontology (GO)
Cellular componentGO:0000328lumen of vacuole with cell cycle-correlated...TAS:SGD.
Molecular functionGO:0004252serine-type endopeptidase activityTAS:SGD.
Biological processGO:0009267cellular response to starvationIMP:SGD.
Biological processGO:0030435sporulationIMP:SGD.
Biological processGO:0007039vacuolar protein catabolic processIMP:SGD.
EC number 3.4.21.48

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
58Phosphoserine.

Sequence Information
Sequence length635
Molecular weight69621

	---------+----------+----------+----------+----------+
MKLENTLFTL GALGSISAAL VIPNLENAAD HHELINKEDH HERPRKVEFT   50
KDDDEEPSDS EDKEHGKFHK KGRKGQDKES PEFNGKRASG SHGSAHEGGK   100
GMKPKHESSN DDDNDDKKKK PHHKGGCHEN KVEEKKMKGK KVKGKKHHEK   150
TLEKGRHHNR LAPLVSTAQF NPDAISKIIP NRYIIVFKRG APQEEIDFHK   200
ENVQQAQLQS VENLSAEDAF FISTKDTSLS TSEAGGIQDS FNIDNLFSGY   250
IGYFTQEIVD LIRQNPLVDF VERDSIVEAT EFDTQNSAPW GLARISHRER   300
LNLGSFNKYL YDDDAGRGVT SYVIDTGVNI NHKDFEKRAI WGKTIPLNDE   350
DLDGNGHGTH CAGTIASKHY GVAKNANVVA VKVLRSNGSG TMSDVVKGVE   400
YAAKAHQKEA QEKKKGFKGS TANMSLGGGK SPALDLAVNA AVEVGIHFAV   450
AAGNENQDAC NTSPASADKA ITVGASTLSD DRAYFSNWGK CVDVFAPGLN   500
ILSTYIGSDD ATATLSGTSM ASPHVAGLLT YFLSLQPGSD SEFFELGQDS   550
LTPQQLKKKL IHYSTKDILF DIPEDTPNVL IYNGGGQDLS AFWNDTKKSH   600
SSGFKQELNM DEFIGSKTDL IFDQVRDILD KLNII                   635