SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePPQ1
Systematic nameYPL179W
AliasSAL6
DescriptionSerine/threonine-protein phosphatase PPQ

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP32945
SGD YPL179W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006417regulation of translationIMP:SGD.
EC number 3.1.3.16

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
208Phosphoserine.

Sequence Information
Sequence length549
Molecular weight61420

	---------+----------+----------+----------+----------+
MRRSPSRSNN NFAVPNCSTN SNSSQQQLTT PSDDLNSNEP NDPDDSRSLP   50
TIKKFNNKHS INNYNTLASA GKNNNNKRAS NDNLLIPGEN AHKQKIYTKD   100
ENLKSLYLDI DVSVAKALSS SATAPKLINT ARTSSTTTAT TSNNILTSPS   150
YRESNYSSPS SYSFSSYYSS ATSASSSTSS FLKSSGLSSR VKSPSSSVKA   200
GSFGAPSSPT SGIPNPKSSK KPIFLRRYSH DTSSNEGLDI DVAIEKLLQV   250
GESREITKTS KKKNFPFHSW EIQLICYHAR EIFLNQPTLL RLQAPIKVVG   300
DVHGQFNDLL RILKLSGVPS DTNYLFLGDY VDRGKNSLET ILLLLCYKIK   350
YKDNFFMLRG NHESANVTKM YGFYDECKRR LSSKVWKMFV DVFNTLPLAA   400
IIQDKIFCVH GGISPDLHDM KQIEKVARPT DIPESGLVTD LLWSDPDPQV   450
TDWSENDRGV SYTFSKRNVL DFCAKFKFDL ILRGHMVVED GYEFFARKKF   500
VTIFSAPNYC GEFHNWGAVM SVTTGMMCSF ELLKPRALKN KKKLYKTKV    549