SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePPN1
Systematic nameYDR452W
AliasPHM5
DescriptionEndopolyphosphatase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1   HECTNARSP5   
2   HECTNARSP5  Vacuolar Degradation
3  UBC4RINGSPRFTUL1  Vacuolar Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidenceUb-conjugate Confirmation
MethodWestern blot
Reference"Bsd2 binds the ubiquitin ligase Rsp5 and mediates the ubiquitination of transmembrane proteins."
Hettema EH, Valdez-Taubas J, Pelham HR.
EMBO J. 23(6):1279-88 (2004) [14988731]
[2]EvidenceLocalization Assay
Methodfluorescence microscopy (GFP (delivery to the interior of the vacuole))
Reference"The ubiquitin ligase Rsp5p is required for modification and sorting of membrane proteins into multivesicular bodies."
Morvan J, Froissard M, Haguenauer-Tsapis R, Urban-Grimal D.
Traffic. 5(5):383-92 (2004) [15086787]
[3]EvidenceLocalization Assay
Methodfluorescence microscopy (GFP (delivery to the interior of the vacuole))
Reference"A transmembrane ubiquitin ligase required to sort membrane proteins into multivesicular bodies."
Reggiori F, Pelham HR.
Nat Cell Biol. 4(2):117-23 (2002) [11788821]

Cross References
Swiss-Prot accessionQ04119
SGD YDR452W
Gene Ontology (GO)
Cellular componentGO:0005774vacuolar membraneIDA:SGD.
Molecular functionGO:0000298endopolyphosphatase activityIDA:SGD.
Biological processGO:0006797polyphosphate metabolic processIDA:SGD.
EC number 3.6.1.10,3.6.1.11

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length674
Molecular weight78344

	---------+----------+----------+----------+----------+
MVVVGKSEVR NVSMSRPKKK SLIAILSTCV LFFLVFIIGA KFQYVSVFSK   50
FLDDRGDNES LQLLNDIEFT RLGLTPREPV IIKDVKTGKE RKLHGRFLHI   100
TDIHPDPYYV EGSSIDAVCH TGKPSKKKDV APKFGKAMSG CDSPVILMEE   150
TLRWIKENLR DKIDFVIWTG DNIRHDNDRK HPRTEAQIFD MNNIVADKMT   200
ELFSAGNEED PRDFDVSVIP SLGNNDVFPH NMFALGPTLQ TREYYRIWKN   250
FVPQQQQRTF DRSASFLTEV IPGKLAVLSI NTLYLFKANP LVDNCNSKKE   300
PGYQLLLWFG YVLEELRSRG MKVWLSGHVP PIAKNFDQSC YDKFTLWTHE   350
YRDIIIGGLY GHMNIDHFIP TDGKKARKSL LKAMEQSTRV QQGEDSNEED   400
EETELNRILD HAMAAKEVFL MGAKPSNKEA YMNTVRDTYY RKVWNKLERV   450
DEKNVENEKK KKEKKDKKKK KPITRKELIE RYSIVNIGGS VIPTFNPSFR   500
IWEYNITDIV NDSNFAVSEY KPWDEFFESL NKIMEDSLLE DEMDSSNIEV   550
GINREKMGEK KNKKKKKNDK TMPIEMPDKY ELGPAYVPQL FTPTRFVQFY   600
ADLEKINQEL HNSFVESKDI FRYEIEYTSD EKPYSMDSLT VGSYLDLAGR   650
LYENKPAWEK YVEWSFASSG YKDD                               674