SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard namePPH21
Systematic nameYDL134C
DescriptionSerine/threonine-protein phosphatase PP2A-1 catalytic subunit

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP23594
SGD YDL134C
Gene Ontology (GO)
Cellular componentGO:0000159protein phosphatase type 2A complexTAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0000158protein phosphatase type 2A activityTAS:SGD.
Biological processGO:0007015actin filament organizationTAS:SGD.
Biological processGO:0007117budding cell bud growthTAS:SGD.
Biological processGO:0000082G1/S transition of mitotic cell cycleIGI:SGD.
Biological processGO:0007094mitotic spindle checkpointIPI:SGD.
Biological processGO:0006470protein amino acid dephosphorylationTAS:SGD.
Biological processGO:0006412translationIPI:SGD.
EC number 3.1.3.16

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
369Leucine methyl ester.

Sequence Information
Sequence length369
Molecular weight41938

	---------+----------+----------+----------+----------+
MDTDLDVPMQ DAVTEQLTPT VSEDMDLNNN SSDNNAEEFS VDDLKPGSSG   50
IADHKSSKPL ELNNTNINQL DQWIEHLSKC EPLSEDDVAR LCKMAVDVLQ   100
FEENVKPINV PVTICGDVHG QFHDLLELFK IGGPCPDTNY LFMGDYVDRG   150
YYSVETVSYL VAMKVRYPHR ITILRGNHES RQITQVYGFY DECLRKYGSA   200
NVWKMFTDLF DYFPITALVD NKIFCLHGGL SPMIETIDQV RELNRIQEVP   250
HEGPMCDLLW SDPDDRGGWG ISPRGAGFTF GQDVSEQFNH TNDLSLIARA   300
HQLVMEGYAW SHQQNVVTIF SAPNYCYRCG NQAAIMEVDE NHNRQFLQYD   350
PSVRPGEPSV SRKTPDYFL                                     369