SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePOP4
Systematic nameYBR257W
DescriptionRNases MRP/P 32.9 kDa subunit

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP38336
SGD YBR257W
Gene Ontology (GO)
Cellular componentGO:0005655nucleolar ribonuclease P complexIDA:SGD.
Cellular componentGO:0000172ribonuclease MRP complexIDA:SGD.
Biological processGO:0006379mRNA cleavageIPI:SGD.
Biological processGO:0006364rRNA processingIMP:SGD.
Biological processGO:0008033tRNA processingIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length279
Molecular weight32881

	---------+----------+----------+----------+----------+
MDRTQTFIKD CLFTKCLEDP EKPFNENRFQ DTLLLLPTDG GLTSRLQRQQ   50
RKSKLNLDNL QKVSQLESAD KQLEKRDYQR INKNSKIALR EYINNCKKNT   100
KKCLKLAYEN KITDKEDLLH YIEEKHPTIY ESLPQYVDFV PMYKELWINY   150
IKELLNITKN LKTFNGSLAL LKLSMADYNG ALLRVTKSKN KTLIGLQGIV   200
IWDSQKFFIM IVKGNIIDEI KCIPKKGTVF QFEIPISDDD DSALRYSILG   250
DRFKYRSVDR AGRKFKSRRC DDMLYYIQN                          279