SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePOL30
Systematic nameYBR088C
DescriptionProliferating cell nuclear antigen

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K164MULTI(K63-linked)UBC13RINGSPRFRAD5  Error-free Pathway of post-replicational DNA repair
2K164MONORAD6RINGSPRFRAD18  Error-prone Pathway of TLS (Translesion Synthesis) DNA repair
* Blank: not determined, NA: not applicable.

References
[1]EvidenceHigh MW Product Confirmation
MethodWestern blot
Reference"RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO."
Hoege C, Pfander B, Moldovan GL, Pyrowolakis G, Jentsch S.
Nature. 419(6903):135-41 (2002) [12226657]
[2]EvidenceHigh MW Product Confirmation
MethodWestern blot
Reference"RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO."
Hoege C, Pfander B, Moldovan GL, Pyrowolakis G, Jentsch S.
Nature. 419(6903):135-41 (2002) [12226657]

Cross References
Swiss-Prot accessionP15873
SGD YBR088C
Gene Ontology (GO)
Cellular componentGO:0005634nucleusIDA:SGD.
Cellular componentGO:0043626PCNA complexTAS:SGD.
Cellular componentGO:0005657replication forkTAS:SGD.
Molecular functionGO:0030337DNA polymerase processivity factor activityIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006284base-excision repairTAS:SGD.
Biological processGO:0030466chromatin silencing at silent mating-type c...IMP:SGD.
Biological processGO:0006348chromatin silencing at telomereIMP:SGD.
Biological processGO:0006273lagging strand elongationIDA:SGD.
Biological processGO:0006272leading strand elongationIDA:SGD.
Biological processGO:0006298mismatch repairIMP:SGD.
Biological processGO:0007064mitotic sister chromatid cohesionIGI:SGD.
Biological processGO:0006280mutagenesisIGI:SGD.
Biological processGO:0006289nucleotide-excision repairIMP:SGD.
Biological processGO:0006301postreplication repairIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length258
Molecular weight28916

	---------+----------+----------+----------+----------+
MLEAKFEEAS LFKRIIDGFK DCVQLVNFQC KEDGIIAQAV DDSRVLLVSL   50
EIGVEAFQEY RCDHPVTLGM DLTSLSKILR CGNNTDTLTL IADNTPDSII   100
LLFEDTKKDR IAEYSLKLMD IDADFLKIEE LQYDSTLSLP SSEFSKIVRD   150
LSQLSDSINI MITKETIKFV ADGDIGSGSV IIKPFVDMEH PETSIKLEMD   200
QPVDLTFGAK YLLDIIKGSS LSDRVGIRLS SEAPALFQFD LKSGFLQFFL   250
APKFNDEE                                                 258

	* Ubiquitinated lysine is denoted as red color.