SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard namePOB3
Systematic nameYML069W
DescriptionFACT complex subunit POB3

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ04636
SGD YML069W
Gene Ontology (GO)
Cellular componentGO:0035101FACT complexIDA:SGD.
Cellular componentGO:0000790nuclear chromatinIDA:SGD.
Molecular functionGO:0003682chromatin bindingIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006333chromatin assembly or disassemblyIPI:SGD.
Biological processGO:0006338chromatin remodelingIPI:SGD.
Biological processGO:0006261DNA-dependent DNA replicationIPI:SGD.
Biological processGO:0006368RNA elongation from RNA polymerase II promoterIPI:SGD.
Biological processGO:0006367transcription initiation from RNA polymeras...IDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
194Phosphoserine.
195Phosphoserine.

Sequence Information
Sequence length552
Molecular weight62994

	---------+----------+----------+----------+----------+
MSTDFDRIYL NQSKFSGRFR IADSGLGWKI STSGGSAANQ ARKPFLLPAT   50
ELSTVQWSRG CRGYDLKINT KNQGVIQLDG FSQDDYNLIK NDFHRRFNIQ   100
VEQREHSLRG WNWGKTDLAR NEMVFALNGK PTFEIPYARI NNTNLTSKNE   150
VGIEFNIQDE EYQPAGDELV EMRFYIPGVI QTNVDENMTK KEESSNEVVP   200
KKEDGAEGED VQMAVEEKSM AEAFYEELKE KADIGEVAGD AIVSFQDVFF   250
TTPRGRYDID IYKNSIRLRG KTYEYKLQHR QIQRIVSLPK ADDIHHLLVL   300
AIEPPLRQGQ TTYPFLVLQF QKDEETEVQL NLEDEDYEEN YKDKLKKQYD   350
AKTHIVLSHV LKGLTDRRVI VPGEYKSKYD QCAVSCSFKA NEGYLYPLDN   400
AFFFLTKPTL YIPFSDVSMV NISRAGQTST SSRTFDLEVV LRSNRGSTTF   450
ANISKEEQQL LEQFLKSKNL RVKNEDREVQ ERLQTALGSD SDEEDINMGS   500
AGEDDESVDE DFQVSSDNDA DEVAEEFDSD AALSDAEGGS DEERPSKKPK   550
VE                                                       552