SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard namePNG1
Systematic nameYPL096W
DescriptionPeptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]

Cross References
Swiss-Prot accessionQ02890
SGD YPL096W
Gene Ontology (GO)
Cellular componentGO:0005829cytosolIDA:SGD.
Cellular componentGO:0005739mitochondrionIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0000224peptide-N4-(N-acetyl-beta-glucosaminyl)aspa...IDA:SGD.
Biological processGO:0006515misfolded or incompletely synthesized prote...IMP:SGD.
Biological processGO:0006517protein deglycosylationIDA:SGD.
EC number 3.5.1.52

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length363
Molecular weight42485

	---------+----------+----------+----------+----------+
MGEVYEKNNI DFDSIAKMLL IKYKDFILSK FKKAAPVENI RFQNLVHTNQ   50
FAQGVLGQSQ HLCTVYDNPS WHSIVLETLD LDLIYKNVDK EFAKDGHAEG   100
ENIYTDYLVK ELLRYFKQDF FKWCNKPDCN HCGQNTSENM TPLGSQGPNG   150
EESKFNCGTV EIYKCNRCGN ITRFPRYNDP IKLLETRKGR CGEWCNLFTL   200
ILKSFGLDVR YVWNREDHVW CEYFSNFLNR WVHVDSCEQS FDQPYIYSIN   250
WNKKMSYCIA FGKDGVVDVS KRYILQNELP RDQIKEEDLK FLCQFITKRL   300
RYSLNDDEIY QLACRDEQEQ IELIRGKTQE TKSESVSAAS KSSNRGRESG   350
SADWKAQRGE DGK                                           363