SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePMT4
Systematic nameYJR143C
DescriptionDolichyl-phosphate-mannose--protein mannosyltransferase 4

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP46971
SGD YJR143C
Gene Ontology (GO)
Cellular componentGO:0005783endoplasmic reticulumTAS:SGD.
Molecular functionGO:0004169dolichyl-phosphate-mannose-protein mannosyl...IMP:SGD.
Molecular functionGO:0042802identical protein bindingIPI:IntAct.
Biological processGO:0006493protein amino acid O-linked glycosylationIMP:SGD.
EC number 2.4.1.109

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length762
Molecular weight87966

	---------+----------+----------+----------+----------+
MSVPKKRNHG KLPPSTKDVD DPSLKYTKAA PKCEQVAEHW LLQPLPEPES   50
RYSFWVTIVT LLAFAARFYK IWYPKEVVFD EVHFGKFASY YLERSYFFDV   100
HPPFAKMMIA FIGWLCGYDG SFKFDEIGYS YETHPAPYIA YRSFNAILGT   150
LTVPIMFNTL KELNFRAITC AFASLLVAID TAHVTETRLI LLDAILIISI   200
AATMYCYVRF YKCQLRQPFT WSWYIWLHAT GLSLSFVIST KYVGVMTYSA   250
IGFAAVVNLW QLLDIKAGLS LRQFMRHFSK RLNGLVLIPF VIYLFWFWVH   300
FTVLNTSGPG DAFMSAEFQE TLKDSPLSVD SKTVNYFDII TIKHQDTDAF   350
LHSHLARYPQ RYEDGRISSA GQQVTGYTHP DFNNQWEVLP PHGSDVGKGQ   400
AVLLNQHIRL RHVATDTYLL AHDVASPFYP TNEEITTVTL EEGDGELYPE   450
TLFAFQPLKK SDEGHVLKSK TVSFRLFHVD TSVALWTHND ELLPDWGFQQ   500
QEINGNKKVI DPSNNWVVDE IVNLDEVRKV YIPKVVKPLP FLKKWIETQK   550
SMFEHNNKLS SEHPFASEPY SWPGSLSGVS FWTNGDEKKQ IYFIGNIIGW   600
WFQVISLAVF VGIIVADLIT RHRGYYALNK MTREKLYGPL MFFFVSWCCH   650
YFPFFLMARQ KFLHHYLPAH LIACLFSGAL WEVIFSDCKS LDLEKDEDIS   700
GASYERNPKV YVKPYTVFLV CVSCAVAWFF VYFSPLVYGD VSLSPSEVVS   750
REWFDIELNF SK                                            762