SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePMA2
Systematic nameYPL036W
DescriptionPlasma membrane ATPase 2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP19657
SGD YPL036W
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Cellular componentGO:0005886plasma membraneIDA:SGD.
Molecular functionGO:0008553hydrogen-exporting ATPase activity, phospho...IDA:SGD.
Biological processGO:0015992proton transportIDA:SGD.
Biological processGO:0006885regulation of pHIEP:SGD.
EC number 3.6.3.6

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
536Phosphoserine.
926Phosphothreonine.
941Phosphothreonine.

Sequence Information
Sequence length947
Molecular weight102172

	---------+----------+----------+----------+----------+
MSSTEAKQYK EKPSKEYLHA SDGDDPANNS AASSSSSSST STSASSSAAA   50
VPRKAAAASA ADDSDSDEDI DQLIDELQSN YGEGDESGEE EVRTDGVHAG   100
QRVVPEKDLS TDPAYGLTSD EVARRRKKYG LNQMAEENES LIVKFLMFFV   150
GPIQFVMEAA AILAAGLSDW VDVGVICALL LLNASVGFIQ EFQAGSIVDE   200
LKKTLANTAT VIRDGQLIEI PANEVVPGEI LQLESGTIAP ADGRIVTEDC   250
FLQIDQSAIT GESLAAEKHY GDEVFSSSTV KTGEAFMVVT ATGDNTFVGR   300
AAALVGQASG VEGHFTEVLN GIGIILLVLV IATLLLVWTA CFYRTVGIVS   350
ILRYTLGITI IGVPVGLPAV VTTTMAVGAA YLAKKQAIVQ KLSAIESLAG   400
VEILCSDKTG TLTKNKLSLH EPYTVEGVSP DDLMLTACLA ASRKKKGLDA   450
IDKAFLKSLI EYPKAKDALT KYKVLEFHPF DPVSKKVTAV VESPEGERIV   500
CVKGAPLFVL KTVEEDHPIP EDVHENYENK VAELASRGFR ALGVARKRGE   550
GHWEILGVMP CMDPPRDDTA QTINEARNLG LRIKMLTGDA VGIAKETCRQ   600
LGLGTNIYNA ERLGLGGGGD MPGSELADFV ENADGFAEVF PQHKYRVVEI   650
LQNRGYLVAM TGDGVNDAPS LKKADTGIAV EGATDAARSA ADIVFLAPGL   700
SAIIDALKTS RQIFHRMYSY VVYRIALSLH LEIFLGLWIA ILNNSLDINL   750
IVFIAIFADV ATLTIAYDNA PYAPEPVKWN LPRLWGMSII LGIVLAIGSW   800
ITLTTMFLPN GGIIQNFGAM NGVMFLQISL TENWLIFVTR AAGPFWSSIP   850
SWQLAGAVFA VDIIATMFTL FGWWSENWTD IVSVVRVWIW SIGIFCVLGG   900
FYYIMSTSQA FDRLMNGKSL KEKKSTRSVE DFMAAMQRVS TQHEKSS      947