SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePIN3
Systematic nameYPR154W
AliasLSB2
Description[PSI+] inducibility protein 3

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K80        
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ06449
SGD YPR154W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0030036actin cytoskeleton organization and biogenesisIPI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
52Phosphoserine.

Sequence Information
Sequence length215
Molecular weight23539

	---------+----------+----------+----------+----------+
MSASLINRSL TNIRTELDFL KGSNVISNDV YDQINKSLPA KWDPANAPRN   50
ASPASLEYVE ALYQFDPQQD GDLGLKPGDK VQLLEKLSPE WYKGSCNGRT   100
GIFPANYVKP AFSGSNGPSN LPPPPQYKAQ ELQQIPTQNS AASSYQQQPF   150
PPPSTNYYQQ PQQQPQQAPP PQQQQQQQQH QSSHSHLKSF GSKLGNAAIF   200
GAGASIGSDI VNNIF                                         215

	* Ubiquitinated lysine is denoted as red color.