SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePIL1
Systematic nameYGR086C
DescriptionSphingolipid long chain base-responsive protein PIL1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP53252
SGD YGR086C
Gene Ontology (GO)
Cellular componentGO:0032126eisosomeIDA:SGD.
Cellular componentGO:0005741mitochondrial outer membraneIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0004860protein kinase inhibitor activityIMP:SGD.
Biological processGO:0006897endocytosisIGI:SGD.
Biological processGO:0008104protein localizationIMP:SGD.
Biological processGO:0009408response to heatIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
41Phosphoserine.
59Phosphoserine.
163Phosphoserine.
230Phosphoserine.
233Phosphothreonine.

Sequence Information
Sequence length339
Molecular weight38349

	---------+----------+----------+----------+----------+
MHRTYSLRNS RAPTASQLQN PPPPPSTTKG RFFGKGGLAY SFRRSAAGAF   50
GPELSRKLSQ LVKIEKNVLR SMELTANERR DAAKQLSIWG LENDDDVSDI   100
TDKLGVLIYE VSELDDQFID RYDQYRLTLK SIRDIEGSVQ PSRDRKDKIT   150
DKIAYLKYKD PQSPKIEVLE QELVRAEAES LVAEAQLSNI TRSKLRAAFN   200
YQFDSIIEHS EKIALIAGYG KALLELLDDS PVTPGETRPA YDGYEASKQI   250
IIDAESALNE WTLDSAQVKP TLSFKQDYED FEPEEGEEEE EEDGQGRWSE   300
DEQEDGQIEE PEQEEEGAVE EHEQVGHQQS ESLPQQTTA               339