SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePIB1
Systematic nameYDR313C
DescriptionE3 ubiquitin-protein ligase PIB1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ06651
SGD YDR313C
Gene Ontology (GO)
Cellular componentGO:0005770late endosomeIDA:SGD.
Cellular componentGO:0000329membrane of vacuole with cell cycle-correla...IDA:SGD.
Molecular functionGO:0032266phosphatidylinositol 3-phosphate bindingIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0004842ubiquitin-protein ligase activityIDA:SGD.
Biological processGO:0016567protein ubiquitinationIDA:SGD.
EC number 6.3.2.-

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length286
Molecular weight32675

	---------+----------+----------+----------+----------+
MVIKEDCINN LARWQADEEA HSCFQCKTNF SFLVRRHHCR CCGRIFCSSC   50
TENFVNYNKK RVHALQKKNS DVESPPYRTC NECYDNLLHL NLLVSSTNRD   100
VRLSQTSVPP NALALSAPDS NTDEDAEILE DSVDQSGTAC RSEESSQNEE   150
DHFCPICNSD LTQFPDEEET RKHVEDCIQR AENAQQHTNT SDAADDSVKE   200
SPAFQNRMLV YKISPNTTDN AIKECPICFE NMEPGEKVGR LECLCVFHYK   250
CIKNWFHKRA QMTAAQKGNG HAFVKRNFCP FHDAVF                  286