SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePHO84
Systematic nameYML123C
DescriptionInorganic phosphate transporter PHO84

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K6;K298        
2 MULTI       
3        Proteasomal Degradation
4K298        
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[3]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[4]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP25297
SGD YML123C
Gene Ontology (GO)
Cellular componentGO:0005887integral to plasma membraneIDA:SGD.
Molecular functionGO:0005315inorganic phosphate transporter activityIDA:SGD.
Molecular functionGO:0005384manganese ion transporter activityIMP:SGD.
Biological processGO:0006828manganese ion transportIMP:SGD.
Biological processGO:0006817phosphate transportIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
302Phosphothreonine.
303Phosphoserine.
316Phosphoserine.
317Phosphothreonine.
321Phosphoserine.
577Phosphoserine.
578Phosphoserine.
579Phosphoserine.
581Phosphoserine.

Sequence Information
Sequence length587
Molecular weight64382

	---------+----------+----------+----------+----------+
MSSVNKDTIH VAERSLHKEH LTEGGNMAFH NHLNDFAHIE DPLERRRLAL   50
ESIDDEGFGW QQVKTISIAG VGFLTDSYDI FAINLGITMM SYVYWHGSMP   100
GPSQTLLKVS TSVGTVIGQF GFGTLADIVG RKRIYGMELI IMIVCTILQT   150
TVAHSPAINF VAVLTFYRIV MGIGIGGDYP LSSIITSEFA TTKWRGAIMG   200
AVFANQAWGQ ISGGIIALIL VAAYKGELEY ANSGAECDAR CQKACDQMWR   250
ILIGLGTVLG LACLYFRLTI PESPRYQLDV NAKLELAAAA QEQDGEKKIH   300
DTSDEDMAIN GLERASTAVE SLDNHPPKAS FKDFCRHFGQ WKYGKILLGT   350
AGSWFTLDVA FYGLSLNSAV ILQTIGYAGS KNVYKKLYDT AVGNLILICA   400
GSLPGYWVSV FTVDIIGRKP IQLAGFIILT ALFCVIGFAY HKLGDHGLLA   450
LYVICQFFQN FGPNTTTFIV PGECFPTRYR STAHGISAAS GKVGAIIAQT   500
ALGTLIDHNC ARDGKPTNCW LPHVMEIFAL FMLLGIFTTL LIPETKRKTL   550
EEINELYHDE IDPATLNFRN KNNDIESSSP SQLQHEA                 587

	* Ubiquitinated lysine is denoted as red color.