SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePHO13
Systematic nameYDL236W
Description4-nitrophenylphosphatase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K136        
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP19881
SGD YDL236W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:00038694-nitrophenylphosphatase activityIDA:SGD.
Molecular functionGO:0004035alkaline phosphatase activityIDA:SGD.
Biological processGO:0016576histone dephosphorylationIDA:SGD.
EC number 3.1.3.41

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
198Phosphothreonine.

Sequence Information
Sequence length312
Molecular weight34625

	---------+----------+----------+----------+----------+
MTAQQGVPIK ITNKEIAQEF LDKYDTFLFD CDGVLWLGSQ ALPYTLEILN   50
LLKQLGKQLI FVTNNSTKSR LAYTKKFASF GIDVKEEQIF TSGYASAVYI   100
RDFLKLQPGK DKVWVFGESG IGEELKLMGY ESLGGADSRL DTPFDAAKSP   150
FLVNGLDKDV SCVIAGLDTK VNYHRLAVTL QYLQKDSVHF VGTNVDSTFP   200
QKGYTFPGAG SMIESLAFSS NRRPSYCGKP NQNMLNSIIS AFNLDRSKCC   250
MVGDRLNTDM KFGVEGGLGG TLLVLSGIET EERALKISHD YPRPKFYIDK   300
LGDIYTLTNN EL                                            312

	* Ubiquitinated lysine is denoted as red color.