SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePGI1
Systematic nameYBR196C
DescriptionGlucose-6-phosphate isomerase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MONO HECTNARSP5   
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidenceUb-conjugate Confirmation
Methodluminescent assay (biotin-ubiquitin, GST-substrate)
Reference"A high throughput screen to identify substrates for the ubiquitin ligase Rsp5."
Kus B, Gajadhar A, Stanger K, Cho R, Sun W, Rouleau N, Lee T, Chan D, Wolting C, Edwards A, Bosse R, Rotin D.
J Biol Chem. 280(33):29470-8 (2005) [15955809]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP12709
SGD YBR196C
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0004347glucose-6-phosphate isomerase activityIMP:SGD.
Biological processGO:0006094gluconeogenesisIMP:SGD.
Biological processGO:0006096glycolysisIMP:SGD.
Biological processGO:0006098pentose-phosphate shuntIMP:SGD.
EC number 5.3.1.9

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
2N-acetylserine.
33Phosphoserine.
193Phosphoserine.
198Phosphothreonine.
534Phosphoserine.
537Phosphoserine.

Sequence Information
Sequence length554
Molecular weight61299

	---------+----------+----------+----------+----------+
MSNNSFTNFK LATELPAWSK LQKIYESQGK TLSVKQEFQK DAKRFEKLNK   50
TFTNYDGSKI LFDYSKNLVN DEIIAALIEL AKEANVTGLR DAMFKGEHIN   100
STEDRAVYHV ALRNRANKPM YVDGVNVAPE VDSVLKHMKE FSEQVRSGEW   150
KGYTGKKITD VVNIGIGGSD LGPVMVTEAL KHYAGVLDVH FVSNIDGTHI   200
AETLKVVDPE TTLFLIASKT FTTAETITNA NTAKNWFLSK TGNDPSHIAK   250
HFAALSTNET EVAKFGIDTK NMFGFESWVG GRYSVWSAIG LSVALYIGYD   300
NFEAFLKGAE AVDNHFTQTP LEDNIPLLGG LLSVWYNNFF GAQTHLVAPF   350
DQYLHRFPAY LQQLSMESNG KSVTRGNVFT DYSTGSILFG EPATNAQHSF   400
FQLVHQGTKL IPSDFILAAQ SHNPIENKLH QKMLASNFFA QAEALMVGKD   450
EEQVKAEGAT GGLVPHKVFS GNRPTTSILA QKITPATLGA LIAYYEHVTF   500
TEGAIWNINS FDQWGVELGK VLAKVIGKEL DNSSTISTHD ASTNGLINQF   550
KEWM                                                     554