SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePEX14
Systematic nameYGL153W
DescriptionPeroxisomal membrane protein PEX14

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP53112
SGD YGL153W
Gene Ontology (GO)
Cellular componentGO:0005778peroxisomal membraneIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0016558protein import into peroxisome matrixIPI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length341
Molecular weight38420

	---------+----------+----------+----------+----------+
MSDVVSKDRK ALFDSAVSFL KDESIKDAPL LKKIEFLKSK GLTEKEIEIA   50
MKEPKKDGIV GDEVSKKIGS TENRASQDMY LYEAMPPTLP HRDWKDYFVM   100
ATATAGLLYG AYEVTRRYVI PNILPEAKSK LEGDKKEIDD QFSKIDTVLN   150
AIEAEQAEFR KKESETLKEL SDTIAELKQA LVQTTRSREK IEDEFRIVKL   200
EVVNMQNTID KFVSDNDGMQ ELNNIQKEME SLKSLMNNRM ESGNAQDNRL   250
FSISPNGIPG IDTIPSASEI LAKMGMQEES DKEKENGSDA NKDDNAVPAW   300
KKAREQTIDS NASIPEWQKN TAANEISVPD WQNGQVEDSI P            341