SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePET10
Systematic nameYKR046C
DescriptionProtein PET10

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K10        
2        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP36139
SGD YKR046C
Gene Ontology (GO)
Cellular componentGO:0005811lipid particleIDA:SGD.
Biological processGO:0009060aerobic respirationIEP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
2N-acetylserine.

Sequence Information
Sequence length283
Molecular weight31246

	---------+----------+----------+----------+----------+
MSESSISSSK PSVELPQATW SHLQRYPALS KFIKYAESLP PVERLISFNL   50
VVLGSVNQWV SESSSSPRLV KQVVAAGKEG AFKLDELVNL LVFKEGVDGL   100
LYNWKSHSNT PGIWLVWFFV DYVANISNTL LREFLIKPLH LQGSTASKEI   150
GSSGEENKVT DASSLPHVAE LSSTTRGMSQ EIQSKVKSNY IDPTKDLAKE   200
KYDAIVKPTT DKLQSVYIDP TKTKLNETYQ RFTTVYENNL SKSESVPKAI   250
VSTGLDLGNA TIEKLKASRE DQTNSKPAAV STN                     283

	* Ubiquitinated lysine is denoted as red color.