SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePDX3
Systematic nameYBR035C
DescriptionPyridoxamine 5'-phosphate oxidase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K29        
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP38075
SGD YBR035C
Gene Ontology (GO)
Molecular functionGO:0004733pyridoxamine-phosphate oxidase activityIMP:SGD.
Biological processGO:0006631fatty acid metabolic processIMP:SGD.
Biological processGO:0000723telomere maintenanceIMP:SGD.
EC number 1.4.3.5

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length228
Molecular weight26908

	---------+----------+----------+----------+----------+
MTKQAEETQK PIIFAPETYQ YDKFTLNEKQ LTDDPIDLFT KWFNEAKEDP   50
RETLPEAITF SSAELPSGRV SSRILLFKEL DHRGFTIYSN WGTSRKAHDI   100
ATNPNAAIVF FWKDLQRQVR VEGITEHVNR ETSERYFKTR PRGSKIGAWA   150
SRQSDVIKNR EELDELTQKN TERFKDAEDI PCPDYWGGLR IVPLEIEFWQ   200
GRPSRLHDRF VYRRKTENDP WKVVRLAP                           228

	* Ubiquitinated lysine is denoted as red color.