SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePDS1
Systematic nameYDR113C
DescriptionSecurin

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1   RINGAPCCDC20   
2   RINGAPCCDC20   
3   RINGAPCAMA1   
* Blank: not determined, NA: not applicable.

References
[1]EvidenceProtein Stability Assay
MethodWestern blot
Reference"CDC20 and CDH1: a family of substrate-specific activators of APC-dependent proteolysis."
Visintin R, Prinz S, Amon A.
Science. 278(5337):460-3 (1997) [9334304]
[2]EvidenceInteraction Assay
Methodwith substrate receptor
Reference"The anaphase inhibitor Pds1 binds to the APC/C-associated protein Cdc20 in a destruction box-dependent manner."
Hilioti Z, Chung YS, Mochizuki Y, Hardy CF, Cohen-Fix O.
Curr Biol. 11(17):1347-52 (2001) [11553328]
[3]EvidenceHigh MW Product Confirmation
Methodfluorography (in vitro)
Reference"The yeast APC/C subunit Mnd2 prevents premature sister chromatid separation triggered by the meiosis-specific APC/C-Ama1."
Oelschlaegel T, Schwickart M, Matos J, Bogdanova A, Camasses A, Havlis J, Shevchenko A, Zachariae W.
Cell. 120(6):773-88 (2005) [15797379]

Cross References
Swiss-Prot accessionP40316
SGD YDR113C
Gene Ontology (GO)
Cellular componentGO:0005634nucleusIDA:SGD.
Cellular componentGO:0005819spindleIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0000070mitotic sister chromatid segregationIMP:SGD.
Biological processGO:0006606protein import into nucleusIMP:SGD.
Biological processGO:0000725recombinational repairIGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
37Phosphoserine.
139Phosphoserine.
185Phosphoserine.
186Phosphoserine.
277Phosphoserine.
292Phosphoserine, by CDC28 (Probable).

Sequence Information
Sequence length373
Molecular weight41838

	---------+----------+----------+----------+----------+
MMPANEDKEN NIVYTGNESS GINFPQTPAH LLKRSHSNIL KPPVRLDQLK   50
RDANSNNGNT LKYIQGGKEV SPTKRLHTHA QQQGRLPLAA KDNNRSKSFI   100
FPETSNQSKD ADLPQLQNTL SIRKNDQLRK LSQISRSRSR ANHNDLLSNS   150
RKLQKYGSVL GYNALPKMKS LVLKDLADSG KNEESSDDDE GNEDSESKLG   200
KKLQSALLKQ DSSDGENELN GGLGLFNEQG GLQQLIKNST KNEQKTKNDK   250
SDKTDDYDIE IAPQRQEPLP YVPEGYSPFQ QDDIEKLKTF NSPYKLDLED   300
EDDTPDKVDL LPLEQIDEEG EKDETECITR NQEEGAALPL LSKNFKEVAA   350
VPTMELVYSE EGLDPEELED LVT                                373