SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePDR5
Systematic nameYOR153W
AliasLEM1, STS1, YDR1
DescriptionPleiotropic ABC efflux transporter of multiple drugs

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2K825        
* Blank: not determined, NA: not applicable.

References
[1]EvidenceUb-conjugate Confirmation
MethodWestern blot
Reference"The yeast multidrug transporter Pdr5 of the plasma membrane is ubiquitinated prior to endocytosis and degradation in the vacuole."
Egner R, Kuchler K.
FEBS Lett. 378(2):177-81 (1996) [8549828]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
purification (6xHis-Myc-ubiquitin), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]

"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery."
Hitchcock AL, Auld K, Gygi SP, Silver PA.
Proc Natl Acad Sci U S A. 100(22):12735-40 (2003) [14557538]

Cross References
Swiss-Prot accessionP33302
SGD YOR153W
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Cellular componentGO:0005886plasma membraneIDA:SGD.
Molecular functionGO:0008559xenobiotic-transporting ATPase activityTAS:SGD.
Biological processGO:0015893drug transportTAS:SGD.
Biological processGO:0009636response to toxinIEP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
49Phosphothreonine.
54Phosphoserine.
61Phosphoserine.
837Phosphoserine.
840Phosphoserine.
841Phosphoserine.
849Phosphoserine.
850Phosphoserine.
854Phosphoserine.
857Phosphotyrosine.
863Phosphoserine.

Sequence Information
Sequence length1511
Molecular weight170438

	---------+----------+----------+----------+----------+
MPEAKLNNNV NDVTSYSSAS SSTENAADLH NYNGFDEHTE ARIQKLARTL   50
TAQSMQNSTQ SAPNKSDAQS IFSSGVEGVN PIFSDPEAPG YDPKLDPNSE   100
NFSSAAWVKN MAHLSAADPD FYKPYSLGCA WKNLSASGAS ADVAYQSTVV   150
NIPYKILKSG LRKFQRSKET NTFQILKPMD GCLNPGELLV VLGRPGSGCT   200
TLLKSISSNT HGFDLGADTK ISYSGYSGDD IKKHFRGEVV YNAEADVHLP   250
HLTVFETLVT VARLKTPQNR IKGVDRESYA NHLAEVAMAT YGLSHTRNTK   300
VGNDIVRGVS GGERKRVSIA EVSICGSKFQ CWDNATRGLD SATALEFIRA   350
LKTQADISNT SATVAIYQCS QDAYDLFNKV CVLDDGYQIY YGPADKAKKY   400
FEDMGYVCPS RQTTADFLTS VTSPSERTLN KDMLKKGIHI PQTPKEMNDY   450
WVKSPNYKEL MKEVDQRLLN DDEASREAIK EAHIAKQSKR ARPSSPYTVS   500
YMMQVKYLLI RNMWRLRNNI GFTLFMILGN CSMALILGSM FFKIMKKGDT   550
STFYFRGSAM FFAILFNAFS SLLEIFSLYE ARPITEKHRT YSLYHPSADA   600
FASVLSEIPS KLIIAVCFNI IFYFLVDFRR NGGVFFFYLL INIVAVFSMS   650
HLFRCVGSLT KTLSEAMVPA SMLLLALSMY TGFAIPKKKI LRWSKWIWYI   700
NPLAYLFESL LINEFHGIKF PCAEYVPRGP AYANISSTES VCTVVGAVPG   750
QDYVLGDDFI RGTYQYYHKD KWRGFGIGMA YVVFFFFVYL FLCEYNEGAK   800
QKGEILVFPR SIVKRMKKRG VLTEKNANDP ENVGERSDLS SDRKMLQESS   850
EEESDTYGEI GLSKSEAIFH WRNLCYEVQI KAETRRILNN VDGWVKPGTL   900
TALMGASGAG KTTLLDCLAE RVTMGVITGD ILVNGIPRDK SFPRSIGYCQ   950
QQDLHLKTAT VRESLRFSAY LRQPAEVSIE EKNRYVEEVI KILEMEKYAD   1000
AVVGVAGEGL NVEQRKRLTI GVELTAKPKL LVFLDEPTSG LDSQTAWSIC   1050
QLMKKLANHG QAILCTIHQP SAILMQEFDR LLFMQRGGKT VYFGDLGEGC   1100
KTMIDYFESH GAHKCPADAN PAEWMLEVVG AAPGSHANQD YYEVWRNSEE   1150
YRAVQSELDW MERELPKKGS ITAAEDKHEF SQSIIYQTKL VSIRLFQQYW   1200
RSPDYLWSKF ILTIFNQLFI GFTFFKAGTS LQGLQNQMLA VFMFTVIFNP   1250
ILQQYLPSFV QQRDLYEARE RPSRTFSWIS FIFAQIFVEV PWNILAGTIA   1300
YFIYYYPIGF YSNASAAGQL HERGALFWLF SCAFYVYVGS MGLLVISFNQ   1350
VAESAANLAS LLFTMSLSFC GVMTTPSAMP RFWIFMYRVS PLTYFIQALL   1400
AVGVANVDVK CADYELLEFT PPSGMTCGQY MEPYLQLAKT GYLTDENATD   1450
TCSFCQISTT NDYLANVNSF YSERWRNYGI FICYIAFNYI AGVFFYWLAR   1500
VPKKNGKLSK K                                             1511

	* Ubiquitinated lysine is denoted as red color.