SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePDR12
Systematic nameYPL058C
DescriptionATP-dependent permease PDR12

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K426        
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ02785
SGD YPL058C
Gene Ontology (GO)
Cellular componentGO:0005886plasma membraneIDA:SGD.
Molecular functionGO:0005342organic acid transporter activityIDA:SGD.
Molecular functionGO:0008559xenobiotic-transporting ATPase activityTAS:SGD.
Biological processGO:0015849organic acid transportIDA:SGD.
Biological processGO:0019541propionate metabolic processIEP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
52Phosphoserine.
56Phosphoserine.

Sequence Information
Sequence length1511
Molecular weight171065

	---------+----------+----------+----------+----------+
MSSTDEHIEK DISSRSNHDD DYANSVQSYA ASEGQVDNED LAATSQLSRH   50
LSNILSNEEG IERLESMARV ISHKTKKEMD SFEINDLDFD LRSLLHYLRS   100
RQLEQGIEPG DSGIAFKNLT AVGVDASAAY GPSVEEMFRN IASIPAHLIS   150
KFTKKSDVPL RNIIQNCTGV VESGEMLFVV GRPGAGCSTF LKCLSGETSE   200
LVDVQGEFSY DGLDQSEMMS KYKGYVIYCP ELDFHFPKIT VKETIDFALK   250
CKTPRVRIDK MTRKQYVDNI RDMWCTVFGL RHTYATKVGN DFVRGVSGGE   300
RKRVSLVEAQ AMNASIYSWD NATRGLDAST ALEFAQAIRT ATNMVNNSAI   350
VAIYQAGENI YELFDKTTVL YNGRQIYFGP ADKAVGYFQR MGWVKPNRMT   400
SAEFLTSVTV DFENRTLDIK PGYEDKVPKS SSEFEEYWLN SEDYQELLRT   450
YDDYQSRHPV NETRDRLDVA KKQRLQQGQR ENSQYVVNYW TQVYYCMIRG   500
FQRVKGDSTY TKVYLSSFLI KALIIGSMFH KIDDKSQSTT AGAYSRGGML   550
FYVLLFASVT SLAEIGNSFS SRPVIVKHKS YSMYHLSAES LQEIITEFPT   600
KFVAIVILCL ITYWIPFMKY EAGAFFQYIL YLLTVQQCTS FIFKFVATMS   650
KSGVDAHAVG GLWVLMLCVY AGFVLPIGEM HHWIRWLHFI NPLTYAFESL   700
VSTEFHHREM LCSALVPSGP GYEGISIANQ VCDAAGAVKG NLYVSGDSYI   750
LHQYHFAYKH AWRNWGVNIV WTFGYIVFNV ILSEYLKPVE GGGDLLLYKR   800
GHMPELGTEN ADARTASREE MMEALNGPNV DLEKVIAEKD VFTWNHLDYT   850
IPYDGATRKL LSDVFGYVKP GKMTALMGES GAGKTTLLNV LAQRINMGVI   900
TGDMLVNAKP LPASFNRSCG YVAQADNHMA ELSVRESLRF AAELRQQSSV   950
PLEEKYEYVE KIITLLGMQN YAEALVGKTG RGLNVEQRKK LSIGVELVAK   1000
PSLLLFLDEP TSGLDSQSAW SIVQFMRALA DSGQSILCTI HQPSATLFEQ   1050
FDRLLLLKKG GKMVYFGDIG PNSETLLKYF ERQSGMKCGV SENPAEYILN   1100
CIGAGATASV NSDWHDLWLA SPECAAARAE VEELHRTLPG RAVNDDPELA   1150
TRFAASYMTQ IKCVLRRTAL QFWRSPVYIR AKFFECVACA LFVGLSYVGV   1200
NHSVGGAIEA FSSIFMLLLI ALAMINQLHV FAYDSRELYE VREAASNTFH   1250
WSVLLLCHAA VENFWSTLCQ FMCFICYYWP AQFSGRASHA GFFFFFYVLI   1300
FPLYFVTYGL WILYMSPDVP SASMINSNLF AAMLLFCGIL QPREKMPAFW   1350
RRLMYNVSPF TYVVQALVTP LVHNKKVVCN PHEYNIMDPP SGKTCGEFLS   1400
TYMDNNTGYL VNPTATENCQ YCPYTVQDQV VAKYNVKWDH RWRNFGFMWA   1450
YICFNIAAML ICYYVVRVKV WSLKSVLNFK KWFNGPRKER HEKDTNIFQT   1500
VPGDENKITK K                                             1511

	* Ubiquitinated lysine is denoted as red color.