SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePDI1
Systematic nameYCL043C
AliasMFP1, TRG1
DescriptionProtein disulfide-isomerase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP17967
SGD YCL043C
Gene Ontology (GO)
Cellular componentGO:0005788endoplasmic reticulum lumenIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0003756protein disulfide isomerase activityIDA:SGD.
Molecular functionGO:0015035protein disulfide oxidoreductase activityIDA:SGD.
Biological processGO:0006457protein foldingIMP:SGD.
EC number 5.3.4.1

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length522
Molecular weight58227

	---------+----------+----------+----------+----------+
MKFSAGAVLS WSSLLLASSV FAQQEAVAPE DSAVVKLATD SFNEYIQSHD   50
LVLAEFFAPW CGHCKNMAPE YVKAAETLVE KNITLAQIDC TENQDLCMEH   100
NIPGFPSLKI FKNSDVNNSI DYEGPRTAEA IVQFMIKQSQ PAVAVVADLP   150
AYLANETFVT PVIVQSGKID ADFNATFYSM ANKHFNDYDF VSAENADDDF   200
KLSIYLPSAM DEPVVYNGKK ADIADADVFE KWLQVEALPY FGEIDGSVFA   250
QYVESGLPLG YLFYNDEEEL EEYKPLFTEL AKKNRGLMNF VSIDARKFGR   300
HAGNLNMKEQ FPLFAIHDMT EDLKYGLPQL SEEAFDELSD KIVLESKAIE   350
SLVKDFLKGD ASPIVKSQEI FENQDSSVFQ LVGKNHDEIV NDPKKDVLVL   400
YYAPWCGHCK RLAPTYQELA DTYANATSDV LIAKLDHTEN DVRGVVIEGY   450
PTIVLYPGGK KSESVVYQGS RSLDSLFDFI KENGHFDVDG KALYEEAQEK   500
AAEEADADAE LADEEDAIHD EL                                 522