SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePDC1
Systematic nameYLR044C
DescriptionPyruvate decarboxylase isozyme 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP06169
SGD YLR044C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0004737pyruvate decarboxylase activityIDA:SGD.
Biological processGO:0019655glucose catabolic process to ethanolIDA:SGD.
Biological processGO:0006090pyruvate metabolic processIDA:SGD.
EC number 4.1.1.1,4.1.1.-

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
2N-acetylserine.
223Phosphoserine.
353Phosphothreonine.
509Phosphothreonine.
514Phosphothreonine.

Sequence Information
Sequence length563
Molecular weight61495

	---------+----------+----------+----------+----------+
MSEITLGKYL FERLKQVNVN TVFGLPGDFN LSLLDKIYEV EGMRWAGNAN   50
ELNAAYAADG YARIKGMSCI ITTFGVGELS ALNGIAGSYA EHVGVLHVVG   100
VPSISAQAKQ LLLHHTLGNG DFTVFHRMSA NISETTAMIT DIATAPAEID   150
RCIRTTYVTQ RPVYLGLPAN LVDLNVPAKL LQTPIDMSLK PNDAESEKEV   200
IDTILALVKD AKNPVILADA CCSRHDVKAE TKKLIDLTQF PAFVTPMGKG   250
SIDEQHPRYG GVYVGTLSKP EVKEAVESAD LILSVGALLS DFNTGSFSYS   300
YKTKNIVEFH SDHMKIRNAT FPGVQMKFVL QKLLTTIADA AKGYKPVAVP   350
ARTPANAAVP ASTPLKQEWM WNQLGNFLQE GDVVIAETGT SAFGINQTTF   400
PNNTYGISQV LWGSIGFTTG ATLGAAFAAE EIDPKKRVIL FIGDGSLQLT   450
VQEISTMIRW GLKPYLFVLN NDGYTIEKLI HGPKAQYNEI QGWDHLSLLP   500
TFGAKDYETH RVATTGEWDK LTQDKSFNDN SKIRMIEIML PVFDAPQNLV   550
EQAKLTAATN AKQ                                           563